Vitamin D3 suppresses the androgen-stimulated growth of mouse mammary carcinoma SC-3 cells by transcriptional repression of fibroblast growth factor 8

Journal of Cellular Physiology
Hirotoshi KawataAkira Tanaka

Abstract

Active metabolites of vitamin A and D are well known to act as growth inhibitors in hormone-related prostate and breast cancers. When various concentrations of 1alpha,25-dihydroxyvitamin D3 (vitamin D3), all-trans-retinoic acid (ATRA) and 9-cis retinoic acid (9-cis RA) were examined, the androgen-stimulated growth of mouse mammary carcinoma SC-3 cells was inhibited by vitamin D3 alone in a dose-dependent manner. A flow cytometer analysis showed that vitamin D3 leads SC-3 cells to relative G1-growth arrest after 72 h. Characterization of vitamin D3-responsive genes using an oligonucleotide microarray demonstrated that 220 genes were upregulated at more than threefold, and 84 genes were downregulated to less than one-third, compared with the testosterone-stimulated SC-3 cells. Neither cyclin-dependent kinase inhibitors (CDKIs) nor the antiapoptotic bcl-2 gene were induced in vitamin D3-responsive genes, with the exception of a slight induction of p15(INK4B). Importantly, fgf8 was markedly repressed in response to vitamin D3. The exogenous addition of FGF8 canceled the growth suppression by vitamin D3 in SC-3 cells, suggesting that the repression of fgf8 is an indispensable step in vitamin D3-mediated growth inhibition. In reporte...Continue Reading

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Citations

Aug 29, 2007·Acta Pharmacologica Sinica·JoEllen Welsh
Aug 13, 2008·Endocrine Reviews·Roger BouillonMarie Demay
Jul 18, 2009·World Journal of Gastroenterology : WJG·Kun-Chun Chiang, Tai C Chen
Oct 15, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Mehmet KorkmazBurcu Erbaykent-Tepedelen
May 6, 2009·Journal of Cellular Biochemistry·Maija P ValtaPirkko L Härkönen
Apr 29, 2015·Canadian Journal of Physiology and Pharmacology·Yotaro MatsumotoTai C Chen
Oct 31, 2017·Endocrinology and Metabolism Clinics of North America·Moray J Campbell, Donald L Trump
Jun 8, 2021·Frontiers in Immunology·Fatima A H Al-JaberiCarsten Geisler

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