Vitamin D/VDR signaling inhibits LPS-induced IFNγ and IL-1β in Oral epithelia by regulating hypoxia-inducible factor-1α signaling pathway

Cell Communication and Signaling : CCS
Xuejun GeJie Du

Abstract

Oral lichen planus (OLP) is known as a chronic inflammatory disease. Our recent studies have suggested that vitamin D/vitamin D receptor (VDR) signaling exerts its protective effects on oral keratinocyte apoptosis by regulating microRNA-802 and p53-upregulated modulator of apoptosis (PUMA), but its roles in oral epithelial inflammatory responses in OLP are still unknown. Herein, we identify lipopolysaccharide (LPS) is able to enhance interferon gamma (IFNγ) and interleukin-1 beta (IL-1β) productions in human oral keratinocytes (HOKs) dependent on hypoxia-inducible factor-1α (HIF-1α). HIF-1α and cytokines levels in HOKs were investigated by real-time PCR and western blotting after LPS challenge. The effects of 1,25(OH)2D3 on LPS-induced HIF-1α and cytokines were tested by real-time PCR, western blotting, siRNA-interference and plasmids transfection techniques. The roles of 1,25(OH)2D3 in regulating HIF-1α levels were investigated using western blotting, siRNA-interference, plasmids transfection and Chromatin Immunoprecipitation (ChIP) assays. Finally, HIF-1α, IFNγ and IL-1β expressions in oral epithelia derived from mice and individuals were measured by real-time PCR, western blotting and immunohistochemical staining. As a criti...Continue Reading

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Citations

Aug 2, 2019·Current Opinion in Lipidology·Tamer SallamLinda L Demer
Mar 4, 2021·Journal of Cellular and Molecular Medicine·Xuejun GeJie Du
Mar 25, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Gang XueJie Du
May 13, 2021·Current Developments in Nutrition·Matthew F Warren, Kimberly A Livingston
Aug 13, 2021·Cell Communication and Signaling : CCS·Xiangyu WangJie Du
Nov 19, 2021·Seminars in Liver Disease·Massimiliano CadamuroLuca Fabris

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Methods Mentioned

BETA
genotyping
PCR
ChIP
transfection
biopsies

Software Mentioned

Elisa

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