PMID: 9537838Apr 16, 1998Paper

Vitamin E induces ramification and downregulation of adhesion molecules in cultured microglial cells

Glia
F L HeppnerN P Hailer

Abstract

Microglial cells in the healthy adult CNS possess a characteristic ramified morphology and show little or no expression of major histocompatibility complex (MHC) or adhesion molecules. In contrast, microglial cells isolated from newborn rat brains inevitably show a nonramified amoeboid morphology and express immunoeffector molecules, such as MHC class I and II, and various adhesion molecules thought to be markers of microglial activation. Furthermore, they produce large amounts of oxygen radicals. Treatment of cultured microglial cells with the antioxidants vitamin E (alpha-tocopherol) and vitamin C (ascorbic acid) induced a ramified microglial morphology after 48 h in vitro, otherwise only seen in healthy adult CNS tissue or in co-culture with astrocytes. Morphological transformation of microglial cells was quantified by morphometric analysis and was found to be statistically significant. Ramification of microglia induced by vitamin E was accompanied by downregulated expression of adhesion molecules leukocyte function antigen-1, very late antigen-4, and intercellular adhesion molecule-1, as assessed by FACS analysis and immunocytochemistry. Moreover, cell numbers of microglia treated with vitamin E remained stable within 7 day...Continue Reading

Citations

Apr 26, 2011·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·M KippC Beyer
Jan 8, 2000·Brain Research·T MöllerH Kettenmann
Jul 7, 1999·Neuroscience and Biobehavioral Reviews·E Gahtan, J B Overmier
Apr 3, 2001·Journal of Neuroimmunology·M A WollmerR Mentlein
Nov 28, 2002·Advanced Drug Delivery Reviews·Amy H Moore, M K O'Banion
Jan 25, 2005·Nature Medicine·Frank L HeppnerAdriano Aguzzi
Dec 14, 1999·The European Journal of Neuroscience·C EderR Nitsch
Nov 11, 2006·Journal of Neuroinflammation·Brandy L Wilkinson, Gary E Landreth
Jan 26, 2013·PloS One·Kristin I Løken-AmsrudTrygve Holmøy
Feb 11, 2012·Neurotoxicology·G Jean Harry, Andrew D Kraft
Jul 9, 2010·Neuropathology and Applied Neurobiology·F DehghaniN P Hailer
Dec 5, 2012·Acta Neurologica Scandinavica. Supplementum·Ø TorkildsenT Holmøy
Sep 11, 2013·Neuropathology and Applied Neurobiology·Daisuke NotoSachiko Miyake
Apr 13, 2011·Neurobiology of Aging·Andrew D KraftG Jean Harry
Mar 13, 2014·The Journal of Steroid Biochemistry and Molecular Biology·Pardes Habib, Cordian Beyer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.