Vitamin E succinate induces NAG-1 expression in a p38 kinase-dependent mechanism.

Molecular Cancer Therapeutics
Minsub Shim, Thomas E Eling

Abstract

NAG-1 (nonsteroidal anti-inflammatory drug-activated gene), a member of the transforming growth factor-beta superfamily, is involved in many cellular processes, such as inflammation, apoptosis/survival, and tumorigenesis. Vitamin E succinate (VES) is the succinate derivative of alpha-tocopherol and has antitumorigenic activity in a variety of cell culture and animal models. In the current study, the regulation and role of NAG-1 expression in PC-3 human prostate carcinoma cells by VES was examined. VES treatment induced growth arrest and apoptosis as well as an increase in NAG-1 protein and mRNA levels in a time- and concentration-dependent manner. VES treatment induced nuclear translocation and activation of p38 kinase. Pretreatment with p38 kinase inhibitor blocked the VES-induced increase in NAG-1 protein and mRNA levels, whereas an inhibition of protein kinase C, Akt, c-Jun NH(2)-terminal kinase, or MEK activity had no effect on VES-induced NAG-1 levels. Forced expression of constitutively active MKK6, an upstream kinase for p38, induced an increase in NAG-1 promoter activity, whereas p38 kinase inhibitor blocked MKK6-induced increase in NAG-1 promoter activity. VES treatment resulted in >3-fold increase in the half-life of ...Continue Reading

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Citations

Oct 27, 2010·PloS One·Paola TiberioValentina Appierto
Jul 2, 2011·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Xinye XuWei Gao
Sep 3, 2013·Journal of Cellular Physiology·Koh Meng Aw YongRobert H Getzenberg
Dec 25, 2012·Biochemical and Biophysical Research Communications·Sachie ShimizuThomas E Eling
May 2, 2014·Cancer Research·Francesca BruzzeseMartin Augsten

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