PMID: 9435215Jan 22, 1998Paper

Vitamin K-dependent carboxylation of the carboxylase

Proceedings of the National Academy of Sciences of the United States of America
Kathleen L Berkner, B N Pudota

Abstract

Vitamin K-dependent (VKD) proteins require modification by the VKD-gamma-glutamyl carboxylase, an enzyme that converts clusters of glus to glas in a reaction that requires vitamin K hydroquinone, for their activity. We have discovered that the carboxylase also carboxylates itself in a reaction dependent on vitamin K. When pure human recombinant carboxylase was incubated in vitro with 14CO2 and then analyzed after SDS/PAGE, a radiolabeled band corresponding to the size of the carboxylase was observed. Subsequent gla analysis of in vitro-modified carboxylase by base hydrolysis and HPLC showed that all of the radioactivity could be attributed to gla residues. Quantitation of gla, asp, and glu residues indicated 3 mol gla/mol carboxylase. Radiolabeled gla was acid-labile, confirming its identity, and was not observed if vitamin K was not included in the in vitro reaction. Carboxylase carboxylation also was detected in baculovirus-(carboxylase)-infected insect cells but not in mock-infected insect cells, which do not express endogenous VKD proteins or carboxylase. Finally, we showed that the carboxylase was carboxylated in vivo. Carboxylase was purified from recombinant carboxylase BHK cells cultured in the presence or absence of vi...Continue Reading

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Citations

Aug 18, 2009·Fish Physiology and Biochemistry·Christel KrossøyRobin Ørnsrud
Nov 1, 2011·Journal of Computer-aided Molecular Design·Cheng-Tsung LuTzong-Yi Lee
May 11, 2002·Life Sciences·Zhong-Qian LiBrian I Carr
Jul 14, 2005·Annual Review of Nutrition·Kathleen L Berkner
Mar 6, 2012·BMC Bioinformatics·Tzong-Yi LeeKai-Yao Huang
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·J D KulmanE W Davie
Sep 19, 2008·The Journal of Investigative Dermatology·Qiaoli LiJouni Uitto
Mar 28, 2013·Geriatrics & Gerontology International·Kotaro AzumaSatoshi Inoue
Sep 30, 2014·Biochemical and Biophysical Research Communications·Sachiko ShibaSatoshi Inoue
Dec 1, 2004·JPEN. Journal of Parenteral and Enteral Nutrition·Murray Bern
Jan 13, 2018·JCI Insight·Julie LacombeMathieu Ferron
Jun 20, 2019·International Journal of Molecular Sciences·Kotaro Azuma, Satoshi Inoue
Aug 5, 2000·The Journal of Nutrition·K L Berkner
Feb 3, 2021·Molecular and Cellular Biology·Sachiko ShibaSatoshi Inoue
Feb 17, 2021·Journal of Thrombosis and Haemostasis : JTH·Suvoshree GhoshJohannes Oldenburg
Sep 3, 2021·Nutrition Reviews·Přemysl MladěnkaUNKNOWN OEMONOM researchers and collaborators

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