PMID: 8939773Dec 1, 1996Paper

Vitamin K2 promotes 1alpha,25(OH)2 vitamin D3-induced mineralization in human periosteal osteoblasts

Calcified Tissue International
Y KoshiharaM Shiraki

Abstract

The effect of vitamin K on mineralization by human periosteal osteoblasts was investigated in the absence and presence of 1alpha, 25 dihydroxyvitamin D3 (1,25(OH)2D3). Vitamin K1 and K2, but not vitamin K3, at 2.5 microM enhanced in vitro mineralization when cells were cultured with vitamin K for 20 days after reaching confluence in vitro. Vitamin K2 (2-methyl-3-all-trans-tetraphenyl-1, 4-naphthoquinone : menatetrenone) was the most potent of these vitamin K analogs; it slightly inhibited alkaline phosphatase (ALP) activity. Human osteoblasts were mineralized and showed the enhanced ALP activity on treatment with 10(-9) M of 1,25(OH)2D3 for 20 or 25 days after confluence. Vitamin K2 promoted the 1,25(OH)2D3-induced mineralization, but slightly inhibited the 1,25(OH)2D3-induced ALP activity. Moreover, vitamin K2 enhanced the 1,25(OH)2D3-induced osteocalcin accumulation in the cells and the extracellular matrix (cell layer), but inhibited the osteocalcin content in the medium produced by the 1,25(OH)2D3 treatment. However, vitamin K2 alone did not induce osteocalcin production in the human osteoblasts. On Northern blot analysis, osteocalcin mRNA expression on 1, 25(OH)2D3-treated cells was enhanced by vitamin K2 treatment, but vi...Continue Reading

References

May 1, 1990·The American Journal of Clinical Nutrition·Y UsuiK Ozawa
Jan 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·Y KoshiharaS Higaki
May 1, 1985·Calcified Tissue International·B Auf'mkolkE R Schwartz
Jun 15, 1987·Biochemical and Biophysical Research Communications·Y KoshiharaS Higaki
Sep 1, 1966·The Journal of Nutrition·J T Matschiner, E A Doisy
May 31, 1983·Biochemical and Biophysical Research Communications·Z Deyl, M Adam
Mar 1, 1983·The Journal of Cell Biology·B Ecarot-CharrierG Pereira
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P A PriceW S Jee
Dec 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·P PastoureauP D Delmas
Apr 1, 1993·The Journal of Clinical Investigation·P SzulcP D Delmas
May 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K HosodaY Seino
Aug 1, 1996·Nature·P DucyG Karsenty
Mar 19, 1960·Nature·J H BOUCKAERT, A H SAID

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Citations

Dec 1, 2011·European Journal of Nutrition·Nina EmausVinjar M Fønnebø
Oct 31, 2001·Nutrition·P Weber
Aug 24, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T SugiyamaS Kawai
Apr 6, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M ShirakiM Miura
May 5, 2005·Nutrition Reviews·Natalia A Denisova, Sarah L Booth
Oct 2, 2008·Nutrition Reviews·M Kyla Shea, Sarah L Booth
Mar 13, 2009·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·Y MatsumotoT Sakurai
Mar 17, 2004·The Proceedings of the Nutrition Society·Susanne Bügel
Dec 2, 2015·Experimental and Therapeutic Medicine·Hironobu KatsuyamaMasafumi Tomita
Oct 3, 2006·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Toshiyuki YasuiMinoru Irahara
Dec 21, 2004·Molecular and Cellular Endocrinology·Yosuke KannoOsamu Kozawa
Oct 19, 2007·Journal of Materials Science. Materials in Medicine·K OzekiY Fukui
Oct 2, 2007·Nutrition in Clinical Practice : Official Publication of the American Society for Parenteral and Enteral Nutrition·Debra A Pearson
Apr 30, 2009·Annual Review of Nutrition·Sarah L Booth
Oct 31, 2012·Journal of Tissue Engineering and Regenerative Medicine·A GiganteL Tiano
May 16, 2000·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·T MorishitaY Tsukamoto
Jan 11, 2013·Physiological Reviews·Sascha Kopic, John P Geibel
Oct 9, 2020·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·S H RønnB L Langdahl

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