Vitamins in cell culture media: Stability and stabilization strategies

Biotechnology and Bioengineering
Alisa SchnellbaecherAline Zimmer

Abstract

Nowadays, chemically defined cell culture media (CCM) have replaced serum- and hydrolysate-based media that rely on complex ingredients, such as yeast extracts or peptones. Benefits include a significantly lower lot-to-lot variability, more efficient manufacturing by reduction to essential components, and the ability to exclude components that may negatively influence growth, viability, or productivity. Even though current chemically defined CCMs provide an excellent basis for various mammalian biotechnological processes, vitamin instabilities are known to be a key factor contributing to the variabilities still present in liquid CCM as well as to short storage times. In this review, the chemical degradation pathways and products for the most relevant vitamins for CCM will be discussed, with a focus on the effects of light, oxygen, heat, and other CCM compounds. Different approaches to stabilize vitamins in solution, such as replacement with analogs, encapsulation, or the addition of stabilizing compounds will also be reviewed. While these vitamins and vitamin stabilization approaches are presented here as particular for CCM, the application of these concepts can also be considered relevant for pharmaceutical, medical, and food ...Continue Reading

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Citations

Dec 10, 2019·Biotechnology and Bioengineering·Valentine ChevallierLaetitia Malphettes
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Jul 3, 2021·International Journal of Molecular Sciences·Alisa SchnellbaecherAline Zimmer
Oct 2, 2021·Biotechnology and Bioengineering·Andrew M BiedermannJ Christopher Love

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