Vitellin-binding proteins in the nematode Oscheius tipulae (Nematoda, Rhabditida)

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
João Carlos SerinoCarlos E Winter

Abstract

We describe the first application of a non-radioactive ligand-blotting technique to the characterization of proteins interacting with nematode vitellins. Chromatographically purified vitellins from the free-living nematode Oscheius tipulae were labeled with fluorescein in vitro. Ligand-blotting assays with horseradish peroxidase-conjugated anti-fluorescein antibodies showed that labeled vitellins reacted specifically with a polypeptide of approximately 100 kDa, which we named P100. This polypeptide is a specific worm's vitellin-binding protein that is present only in adult worms. Blots containing purified O. tipulae vitellin preparations showed no detectable signal in the 100 kDa region, ruling out any possibility of yolk polypeptides self-assembling under the conditions used in our assay. Experiments done in the presence of alpha-methyl mannoside ruled out the possibility of vitellins binding to P100 through mannose residues. Triton X-114 fractionation of whole worm extracts showed that P100 is either a membrane protein or has highly hydrophobic regions.

References

Jun 10, 1988·Science·C Kenyon
Feb 28, 1986·Biochimica Et Biophysica Acta·J R Wetterau, D B Zilversmit
Apr 4, 1986·Science·M S Brown, J L Goldstein
May 1, 1974·Genetics·J E Sulston, S Brenner
Mar 1, 1983·Developmental Biology·W J Sharrock
Mar 1, 1983·Developmental Biology·J Kimble, W J Sharrock
Jul 1, 1995·Insect Biochemistry and Molecular Biology·T W SappingtonA S Raikhel
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·C P SchonbaumA P Mahowald
May 1, 1996·Molecular Biology and Evolution·C E WinterT Blumenthal
Aug 6, 1998·Insect Biochemistry and Molecular Biology·T W Sappington, A S Raikhel
Dec 10, 1999·Molecular Biology of the Cell·B Grant, D Hirsh
Mar 2, 2002·Traffic·Hanna Fares, Barth Grant
Mar 8, 2002·Insect Molecular Biology·K H Cho, A S Raikhel
Sep 17, 2002·Insect Molecular Biology·M BownesMalcolm Walkinshaw
Jan 9, 2003·Journal of Lipid Research·M Mahmood HussainPaul Dreizen
Dec 6, 2003·Science·Yukimasa ShibataSiegfried Hekimi
Feb 11, 2005·The Journal of Biological Chemistry·Jeremy A SellersGregory S Shelness
May 14, 2005·Current Opinion in Lipidology·Gregory S Shelness, Aubrey S Ledford
Jun 7, 2005·Journal of Biochemical and Biophysical Methods·Il-Young Ahn, Carlos E Winter
Dec 7, 2006·Journal of Lipid Research·Marcel M W SmolenaarsDick J Van der Horst
Jun 5, 2007·PLoS Computational Biology·Harish ShankaranH Steven Wiley
Aug 19, 2007·Molecular BioSystems·Daniel A BlasioleAlan D Attie
Dec 1, 2007·WormBook : the Online Review of C. Elegans Biology·Karin Kiontke, David H A Fitch
Dec 1, 2007·WormBook : the Online Review of C. Elegans Biology·Marie-Anne K Félix
Dec 1, 2007·WormBook : the Online Review of C. Elegans Biology·Barth D Grant, Miyuki Sato

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