PMID: 16519309Mar 8, 2006Paper

Vitellogenin in black turtle (Chelonia mydas agassizii): purification, partial characterization, and validation of an enzyme-linked immunosorbent assay for its detection

Environmental Toxicology and Chemistry
Itzel Sifuentes-RomeroSusan C Gardner

Abstract

Black turtle plasmatic vitellogenin (VTG) was purified from 17beta-estradiol-induced males using ion-exchange chromatography. The isolated protein was identified as VTG by its glycolipoprotein nature and amino acid sequence homology with other vertebrate VTG. It was characterized as a 500-kDa dimer composed of two identical, 200- to 240-kDa monomers. Polyclonal antibodies raised against black turtle VTG showed high titer and specificity, as demonstrated by enzyme-linked immunosorbent assay and Western blot analysis, respectively. The range of the assay was estimated to be between 15 ng/ml and 2 microg/ml, and the inter- and intra-assay coefficients of variation were 9.4 and 7.3%, respectively. Black turtle antibody cross-reacted with VTG of two other sea turtle species, Caretta caretta (loggerhead) and Eretmochelys imbricata (hawksbill), extending the applicability of the assay as part of a sea turtle health assessment program.

Citations

Jun 26, 2010·Journal of Insect Physiology·Damdinsuren BoldbaatarKozo Fujisaki
Sep 11, 2007·Environmental Pollution·Thomas R RainwaterScott T McMurry
May 30, 2016·Environment International·Kimberly A FinlaysonJason P van de Merwe
Mar 21, 2007·Insect Biochemistry and Molecular Biology·Robert D MitchellR Michael Roe

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