Vitellogenin-like proteins among invertebrate species diversity: potential of proteomic mass spectrometry for biomarker development

Environmental Science & Technology
Guillaume JubeauxArnaud Chaumot

Abstract

Cost-effective methodologies along with cross-species applicability constitute key points for biomarker development in ecotoxicology. With the advent of cheaper affordable genomic techniques and high throughput sequencing, omics tools could facilitate the assessment of effects of environmental contaminants for all taxa biodiversity. We assessed the potential of absolute quantification of proteins using mass spectrometry to develop vitellogenin (Vg)-like protein assays for invertebrates. We used available sequences in public databases to rapidly identify Vg-proteotypic peptides in seven species from different main taxa of protostome invertebrates (mollusk bivalves, crustacean amphipods, branchiopods, copepods and isopods, and insect diptera). Functional validation was performed by comparing proteomic signals from reproductive female tissue samples and negative controls (male or juvenile tissues). In a second part, we demonstrate in gammarids, daphnids, drosophilids, and gastropods that the assay validated in Vg-sequenced species can be applied to Vg-unsequenced species thanks to the evolutionary conservation of Vg-proteotypic peptide motifs. Finally, we discuss the relevance of mass spectrometry for biomarker development (specif...Continue Reading

References

Jun 1, 1997·Molecular Biology and Evolution·S L AdamkewiczC J Bult
Oct 26, 1999·Biochemical and Biophysical Research Communications·A NakamuraS Goto
Feb 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Gro V AmdamStig W Omholt
Oct 11, 2003·Analytical and Bioanalytical Chemistry·Bente M NilsenAnders Goksøyr
Feb 11, 2004·Environmental Toxicology and Chemistry·Peter Carsten von der Ohe, Matthias Liess
Apr 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kevin J PetersonMark A McPeek
Jul 21, 2004·International Review of Cytology·Matthias OetkenJörg Oehlmann
Oct 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Emmanuel J P DouzeryHervé Philippe
Feb 22, 2005·Fish & Shellfish Immunology·Shicui ZhangXiaodong Shi
Jan 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Siri-Christine SeehuusGro V Amdam
Feb 24, 2006·Environmental Toxicology and Chemistry·Valery E ForbesLis Bach
May 13, 2006·Marine Environmental Research·Alin-Mirel Puinean, Jeanette M Rotchell
Nov 30, 2006·Trends in Ecology & Evolution·David BickfordIndraneil Das
Dec 7, 2006·Journal of Lipid Research·Marcel M W SmolenaarsDick J Van der Horst
Mar 19, 2008·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Gabriel MarkovVincent Laudet
Dec 25, 2008·Journal of Proteome Research·Sheng PanTeresa A Brentnall
Apr 17, 2009·Trends in Genetics : TIG·Romain A Studer, Marc Robinson-Rechavi
Jul 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Gabriel V MarkovVincent Laudet
Jul 10, 2010·Environmental Pollution·Corina M CiocanJeanette M Rotchell
Oct 22, 2010·Environmental Toxicology and Chemistry·Malin C CelanderJames R Wheeler
Sep 6, 2011·Nature·Kevin M KocotKenneth M Halanych
Sep 13, 2011·Molecular Immunology·Min LiuShicui Zhang
Dec 7, 2011·Aquatic Toxicology·Mikko Nikinmaa, Kalle T Rytkönen

❮ Previous
Next ❯

Citations

Dec 18, 2013·Aquatic Toxicology·Stephen ShortAlex T Ford
Sep 30, 2014·Environmental Toxicology and Chemistry·Louis-Charles RainvilleDavid Sheehan
Feb 1, 2014·The Analyst·Louis-Charles RainvilleDavid Sheehan
Oct 9, 2019·Proceedings of the National Academy of Sciences of the United States of America·Atsuo IidaKaori Sano
May 24, 2021·The Science of the Total Environment·Huiying GuoJinshao Ye
Oct 28, 2014·Environmental Science & Technology·Judith TrappOlivier Geffard

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.