Vitellogenin transcytosis in follicular cells of the honeybee Apis mellifera and the wasp Polistes simillimus

Protoplasma
Virgínia Teles DohanikJosé Eduardo Serrão

Abstract

Vitellogenin receptor (VgR) is a low-density lipoprotein receptor responsible for the mediated endocytosis of vitellogenin (Vg) during egg formation in insects. The maturing oocyte is enveloped by a follicular epithelium, which has large intercellular spaces during Vg accumulation (patency). However, Vg has been reported in the cytoplasm of follicular cells, indicating that there may be a transcellular route for its transport. This study verified the presence of VgR in the follicular cells of the ovaries of the honeybee Apis mellifera and the wasp Polistes simillimus in order to evaluate if Vg is transported via transcytosis in these insects. Antibodies specific for vitellogenin receptor (anti-VgR), vitellogenin (anti-Vg), and clathrin (anti-Clt) were used for immunolocalization. The results showed the presence of VgR on the apical and basal plasma membranes of follicular cells of the vitellogenic follicles in both species, indicating that VgR may have been transported from the basal to the apical cell domain, followed by its release into the perivitelline space, evidenced by the presence of apical plasma membrane projections containing VgR. Co-localization proved that Vg bind to VgR and that the transport of this protein is me...Continue Reading

References

Jan 1, 1992·Annual Review of Entomology·A S Raikhel, T S Dhadialla
Aug 1, 1970·Journal of Cellular Physiology·L M Anderson, W H Telfer
Jul 1, 1995·Insect Biochemistry and Molecular Biology·T W SappingtonA S Raikhel
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·C P SchonbaumA P Mahowald
Jul 1, 1997·Physiological Reviews·S MukherjeeF R Maxfield
Aug 6, 1998·Insect Biochemistry and Molecular Biology·T W Sappington, A S Raikhel
Feb 26, 2000·Molecular Biology of the Cell·C P SchonbaumA P Mahowald
Apr 1, 1961·The Journal of Biophysical and Biochemical Cytology·W H TELFER
Feb 1, 1964·The Journal of Cell Biology·T F ROTH, K R PORTER
Jun 3, 2005·Anatomia, histologia, embryologia·L C O LisboaL A O Campos
Feb 14, 2007·Insect Biochemistry and Molecular Biology·Muhammad Tufail, Makio Takeda
May 4, 2011·Journal of Insect Physiology·Dihego Oliveira AzevedoJosé Eduardo Serrão
Mar 14, 2014·Nature Reviews. Molecular Cell Biology·George M ChurchRon Weiss
May 10, 2016·PloS One·Santosh Kumar UpadhyayPraveen C Verma
Apr 5, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Chaoshan HanQingyou Xia

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Citations

Dec 29, 2020·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Luanda Medeiros-SantanaJosé Eduardo Serrão
Mar 12, 2021·Development Genes and Evolution·Agustina PascualRolando Rivera Pomar

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