Vitexin induces apoptosis through mitochondrial pathway and PI3K/Akt/mTOR signaling in human non-small cell lung cancer A549 cells

Biological Research
Xiaoli LiuSuxia Luo

Abstract

Currently, the prognosis of patients with non-small cell lung cancer (NSCLC) remains dismal; hence, it is critical to identify effective anti-NSCLC agents with limited side effects. This study aimed to evaluate the therapeutic potential of flavonoid compound vitexin in human NSCLC cells and the underlying mechanisms. The experimental results indicated that vitexin reduced the viability of A549 cells in a dose-dependent manner with nearly no toxicity against normal human bronchial epithelial 16HBE cells. Vitexin also dose-dependently increased A549 cell apoptosis, accompanied by the decreased Bcl-2/Bax ratio and the increased expression of cleaved caspase-3. Moreover, the in vivo anticancer activity of vitexin was further determined in nude mice bearing A549 cells. In addition, vitexin induced the release of cytochrome c from the mitochondria to the cytosol and the loss of mitochondrial membrane potential. Vitexin also significantly reduced the levels of p-PI3K, p-Akt and p-mTOR, and the pro-apoptotic effect of vitexin on A549 cells was partly blocked by SC79, an Akt activator. Accordingly, we believed that vitexin could be used as a potential therapeutic agent for the treatment of NSCLC in the future.

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Citations

Nov 5, 2019·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·T XieQ Huang
Dec 1, 2020·Natural Product Research·Seham S El-HawwaryUsama Ramadan Abdelmohsen
Dec 24, 2019·Seminars in Cancer Biology·Devesh TewariAnupam Bishayee
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Luis Gustavo Saboia PonteFernando Moreira Simabuco
Jun 11, 2021·The Journal of Pharmacy and Pharmacology·Monaj Kumar SarkarVellingiri Vadivel
Jun 17, 2021·Journal of Agricultural and Food Chemistry·Asifa KhanMohammad Askandar Iqbal
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cinzia ForniSimone Beninati

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Methods Mentioned

BETA
flow cytometry
xenograft

Software Mentioned

GraphPad
GraphPad Prism

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.