Vitrification for bovine embryos with low-quality grade

Animal Science Journal = Nihon Chikusan Gakkaihō
Hiroshi TajimiHitoshi Ushijima

Abstract

This study was conducted to examine the utility of vitrification for bovine embryos with low-quality grade, and simple cryoprotectants dilution method for practitioners. In Experiment 1, survival of frozen embryos was compared with that of vitrified embryos using minimum volume cooling (MVC). Then, vitrified embryos were used to confirm the optimum sucrose concentration in Experiment 2. The survival rates of embryos that had been vitrified following diluted cryoprotectants with the one-step in-straw method were compared with those of fresh control embryos in Experiment 3. Frozen-thawed or vitrified-warmed blastocysts were cultured with TCM-199 supplemented with 100 μmol/L beta-mercaptoethanol +5% fetal bovine serum at 38.5°C in an atmosphere of 5% CO2 in air, their survival after 24 hr were compared. The development to term of fair quality in vivo embryos after vitrification was examined in Experiment 4. Results show that survival rates of frozen-thawed embryos were lower (p < .05) than that of vitrified-warmed ones. When vitrified embryos were warmed in 0.3 mol/L sucrose in straws, their survival rate was 100%. The total cell numbers of vitrified-warmed embryos were comparable to those of fresh control embryos. The six calves ...Continue Reading

References

Mar 1, 1975·Biology of Reproduction·B G Brackett, G Oliphant
Aug 26, 1998·Molecular Reproduction and Development·G VajtaH Callesen
Mar 8, 2005·The Journal of Reproduction and Development·Hitoshi Ushijima
Oct 1, 1983·Theriogenology·G M Lindner, R W Wright
Jul 29, 2010·Animal Science Journal = Nihon Chikusan Gakkaihō·Kiyoshi AkiyamaSatoko Matoba
Oct 12, 2010·Biology of Reproduction·Yasuyuki AbeHiroshi Suzuki
Apr 7, 2011·The Journal of Reproduction and Development·Yasushi InabaKei Imai
Jul 11, 2012·Animal Science Journal = Nihon Chikusan Gakkaihō·Tatsuyuki YoshidaHitoshi Ushijima
Nov 28, 2013·Theriogenology·A Arav

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