Vitrification in straws conserves motility features better than spheres in donkey sperm

Reproduction in Domestic Animals = Zuchthygiene
M Diaz-JimenezM Hidalgo

Abstract

Sperm vitrification as alternative to conventional freezing is increasing in popularity in many species. It has been achieved by direct exposure of diluted semen to liquid nitrogen in spheres or straws. Both techniques have been successfully developed, but they had not been compared yet in donkeys. The aim of this study was to compare these two methods of vitrification for donkey semen. Ejaculates from six Andalusian donkeys were collected and extended in Gent without glycerol supplemented with sucrose 0.1 M (Molar). Samples were slowly cooled at 5°C. For vitrification, 30 μl suspensions (spheres) were dropped directly into liquid nitrogen (LN2 ) or filled in covered 0.25 ml straws and then plunged into the LN2 (straws). For warming, straws and spheres were directly immersed in 3 ml of INRA-96 at 43°C. Total (TM, %) and progressive motility (PM, %) were objectively evaluated by computer-assisted sperm analysis and plasma membrane integrity (PMI, %) by epifluorescence microscopy. Results showed the straw method resulted in significantly higher values than spheres for: TM (54.7% ± 10.1 vs. 28.6% ± 6.5) and PM (44.2% ± 9.4 vs. 17.7% ± 6.4), but no significant differences were found between straws or spheres for PMI (31.5 ± 10.7 vs...Continue Reading

References

Apr 12, 2005·Reproductive Biomedicine Online·Vladimir IsachenkoHans van der Ven
Apr 14, 2011·Andrologia·R SánchezE Isachenko
Nov 29, 2013·Animal : an International Journal of Animal Bioscience·I OrtizM Hidalgo
Nov 4, 2016·Reproduction in Domestic Animals = Zuchthygiene·W F SwansonL M Vansandt
Oct 21, 2017·Reproduction in Domestic Animals = Zuchthygiene·M Diaz-JimenezM Hidalgo
Dec 28, 2017·Animal Reproduction Science·M Diaz-JimenezM Hidalgo
Jan 22, 2018·Theriogenology·Loredana ZilliSebastiano Vilella

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Citations

Mar 9, 2020·Reproductive Biology and Endocrinology : RB&E·Yong TaoMarie-Claude Leveille
Aug 23, 2020·Animals : an Open Access Journal From MDPI·Manuel HidalgoJesús Dorado
Oct 19, 2019·Reproduction in Domestic Animals = Zuchthygiene·Cesar ConsuegraManuel Hidalgo
Jan 31, 2021·Animal : an International Journal of Animal Bioscience·M Diaz-JimenezM Hidalgo
Jul 3, 2021·Journal of Clinical Medicine·Kaan Aydos, Oya Sena Aydos

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