Vitrification of bovine oocytes after treatment with cholesterol-loaded methyl-beta-cyclodextrin

Theriogenology
G Horvath, G E Seidel

Abstract

A major site of cryoinjury during cryopreservation of mammalian oocytes is the plasma membrane. Chilling can irreversibly damage plasma membrane integrity during the lipid phase transition that occurs upon cooling. Membranes containing higher cholesterol concentrations are more fluid at lower temperatures and therefore less sensitive to cooling. The purpose of this study was to determine if cryosurvival of vitrified oocytes could be improved by incubation with cholesterol-loaded methyl-beta-cyclodextrin (CLC) prior to vitrification in the presence or absence of fetal calf serum (FCS), and if cholesterol could enter oocytes through cumulus cells and the zona pellucida. Cumulus-enclosed oocytes incubated with various concentrations (0, 0.75 or 1.5 mg/mL) of CLC in the presence of FCS for 25-45 min prior to vitrification did not result in different rates of development after warming of vitrified oocytes, followed by in vitro fertilization. However, there was an increase (P<0.05) in cleavage and number of eight-cell embryos from oocytes preincubated for 1h with 2mg/mL CLC in a chemically defined system and then handled and vitrified in chemically defined media, in comparison to those not exposed to CLC prior to vitrification or to ...Continue Reading

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Citations

Oct 27, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Joseph Saragusty, Amir Arav
Oct 5, 2010·Veterinary Medicine International·Jennifer R Prentice, Muhammad Anzar
Mar 29, 2007·Reproduction, Fertility, and Development·Sergio LeddaSalvatore Naitana
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Jun 15, 2012·Reproduction in Domestic Animals = Zuchthygiene·C DíezE Gómez
Apr 11, 2013·Molecular Reproduction and Development·Guang-Bin ZhouYun-Peng Hou
Aug 13, 2013·Reproduction in Domestic Animals = Zuchthygiene·R Motamedi-MojdehiR Rajabi-Toustani
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Oct 31, 2012·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Gregory M FahyGeny M M Groothuis
Aug 16, 2016·Theriogenology·José Felipe W SprícigoMargot Alves Nunes Dode
Oct 4, 2016·Systems Biology in Reproductive Medicine·Roya Hedayati KashkaTaghi Lashkarbolouki
Nov 25, 2016·Animal Science Journal = Nihon Chikusan Gakkaihō·Elisa Caroline da Silva SantosKazuhiro Kikuchi
Sep 27, 2018·Biopreservation and Biobanking·Sergei AmstislavskyPierre Comizzoli
Jan 1, 2018·Reproduction, Fertility, and Development·Teresa Mogas
May 27, 2020·Scientific Reports·Marcia de Almeida Monteiro Melo FerrazNucharin Songsasen
Jul 31, 2021·Cryobiology·Luca CirinoChiahsin Lin

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