Vitrification of Carotid Artery Segments: An Integrated Study of Thermophysical Events and Functional Recovery Toward Scale-Up for Clinical Applications

Cell Preservation Technology
S BaicuYoed Rabin

Abstract

In recent years, ice-free cryopreservation by vitrification has been demonstrated to provide superior preservation of tissues compared with conventional freezing methods. To date, this has been accomplished almost exclusively for small model systems, whereas cryopreservation of large tissue samples-of a clinically useful size-continues to be hampered by thermomechanical effects that compromise the structure and function of the tissue. Reduction of mechanical stress is an integral condition of successful cryopreservation of large specimens. The current study focuses on the impact of sample size on both the physical events, observed by cryomacroscopy, and on the outcome on tissue function. To this end, the current study sought to address the question of functional recovery of vitrified carotid artery segments, processed as either artery rings (3-4 mm long) or segments (25 mm long) as selected models; the latter model represents a significant increase in sample size for evaluating the effects of vitrification. Tissue vitrification using an 8.4 M cryoprotectant cocktail solution (VS55) was achieved in 1-ml samples by imposing either a high (50-70 °C/min) or a low (2-3 °C/min) cooling rate, between -40°C and -100°C, and a high rewar...Continue Reading

References

Jul 21, 1999·Journal of Biomechanical Engineering·Y RabinN Wolmark
Mar 4, 2000·Nature Biotechnology·Y C SongM J Taylor
Nov 9, 2000·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Y C SongK G Brockbank
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Jun 21, 2006·Cryobiology·Yoed RabinMatthew C Palastro

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Citations

Sep 11, 2007·Annals of Biomedical Engineering·Jorge L Jimenez RiosYoed Rabin
Oct 30, 2008·Experimental Mechanics·Jorge L Jimenez Rios, Yoed Rabin
Jun 23, 2009·Cryobiology·Brian Wowk
Nov 26, 2015·Journal of Biomechanical Engineering·David P EisenbergYoed Rabin
May 31, 2013·Journal of Biomechanical Engineering·Angela SeawrightBumsoo Han
Jan 5, 2010·Organogenesis·Michael J Taylor, Simona Baicu
Jun 27, 2014·The International Journal of Social Psychiatry·Greet WilrycxChijs van Nieuwenhuizen
Sep 3, 2020·Advanced Healthcare Materials·Zonghu HanJohn C Bischof
Jun 21, 2018·Annals of Biomedical Engineering·Navid ManuchehrabadiJohn Bischof
Mar 3, 2017·Science Translational Medicine·Navid ManuchehrabadiJohn C Bischof

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