Vitrification of mature mouse oocytes: improved results following addition of polyethylene glycol to a dimethyl sulfoxide solution

Cryobiology
L O'NeilBarry J Fuller

Abstract

Oocytes have been successfully cryopreserved using rapid and slow freezing procedures; however, variability in the success of replicates has limited its practical application. We have evaluated the potentially beneficial effects of adding 1 mg/ml of the polymer polyethylene glycol (PEG) (M(r) 8000) to a 6 M dimethyl sulfoxide (Me2SO) vitrification solution. Stepwise addition of cryoprotectant, either with or without PEG, was performed at room temperature (19-21 degrees C). Oocytes were then loaded in plastic insemination straws and held in liquid nitrogen vapour at -140 degrees C for 3 min prior to storage in liquid nitrogen. Oocytes were warmed rapidly to room temperature and removal of cryoprotective agent was effected in the presence of 1 M sucrose solution. Viability was assessed by vitro fertilization. Oocytes cryopreserved after exposure to 6 M Me2SO in the absence of PEG showed 60% normality, 80% fertilization, and 55% development to blastocyst, median of 11 replicate experiments (191 oocytes). Individual replicates yield highly variable survival which ranged from 0 to 100%. The addition of PEG significantly improved oocyte normality to 95% (range 76-100%; median of 9 replicate experiments, 301 oocytes). Rates of fertili...Continue Reading

References

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Citations

Jul 16, 2005·Journal of Assisted Reproduction and Genetics·Mojtaba Rezazadeh Valojerdi, Mojdeh Salehnia
Mar 26, 2004·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Michael TuckerJuergen Liebermann
May 1, 2001·Theriogenology·S LeddaS Naitana
Oct 10, 2001·Human Fertility : Journal of the British Fertility Society·G CoticchioA Borini
Aug 22, 2003·Reproductive Biomedicine Online·Andrea BoriniCarlo Flamigni
Nov 4, 2008·Cryobiology·Wen-Xia FanZhan-Feng Cui
Nov 7, 2008·International Journal of Dermatology·Craig G Burkhart, Craig N Burkhart
Jun 16, 2004·European Journal of Obstetrics, Gynecology, and Reproductive Biology·G CoticchioC Flamigni
Apr 20, 2004·Cryobiology·Erik J WoodsJohn K Critser
Mar 25, 2015·Zygote : the Biology of Gametes and Early Embryos·Qian Li, Long-Bo Cui
Jul 16, 2005·Animal Reproduction Science·Yunus Cetin, Ayhan Bastan
Nov 25, 2016·Animal Science Journal = Nihon Chikusan Gakkaihō·Elisa Caroline da Silva SantosKazuhiro Kikuchi
Nov 22, 2002·Biology of Reproduction·Juergen LiebermannMichael J Tucker
Jun 12, 2004·Cell Transplantation·Hesham M El-ShewyEmmanuel C Opara
Feb 10, 2007·Molecular Reproduction and Development·S SuccuS Naitana
Mar 29, 2000·Theriogenology·J M ShawA O Trounson

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