Vitronectin expression in the airways of subjects with asthma and chronic obstructive pulmonary disease

PloS One
Lina M Salazar-PeláezChun Y Seow

Abstract

Vitronectin, a multifunctional glycoprotein, is involved in coagulation, inhibition of the formation of the membrane attack complex (MAC), cell adhesion and migration, wound healing, and tissue remodeling. The primary cellular source of vitronectin is hepatocytes; it is not known whether resident cells of airways produce vitronectin, even though the glycoprotein has been found in exhaled breath condensate and bronchoalveolar lavage from healthy subjects and patients with interstitial lung disease. It is also not known whether vitronectin expression is altered in subjects with asthma and COPD. In this study, bronchial tissue from 7 asthmatic, 10 COPD and 14 control subjects was obtained at autopsy and analyzed by immunohistochemistry to determine the percent area of submucosal glands occupied by vitronectin. In a separate set of experiments, quantitative colocalization analysis was performed on tracheobronchial tissue sections obtained from donor lungs (6 asthmatics, 4 COPD and 7 controls). Vitronectin RNA and protein expressions in bronchial surface epithelium were examined in 12 subjects who undertook diagnostic bronchoscopy. Vitronectin was found in the tracheobronchial epithelium from asthmatic, COPD, and control subjects, a...Continue Reading

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Citations

Jun 7, 2015·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Magnus PaulssonKristian Riesbeck
Dec 19, 2017·Cell and Tissue Research·Gurpreet Kaur Aulakh
Mar 29, 2021·Pharmacology & Therapeutics·Gang LiuPhilip M Hansbro

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Methods Mentioned

BETA
bronchoalveolar lavage
PCR
confocal microscopy
electrophoresis

Software Mentioned

REST ( Relative Expression Software Tool )
GraphPad Prism
Volocity
Lucia
SingleQuot
Volocity 3D Image Analysis
Primer3
LinRegPCR
Fiji

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