Vitronectin in colorectal adenocarcinoma--synthesis by stromal cells in culture

Experimental Cell Research
B R Tomasini-JohanssonK Rubin

Abstract

We have investigated the expression and cellular source of vitronectin in colorectal adenocarcinoma. Immunofluorescence staining of tissue sections revealed the presence of vitronectin in the stroma of the 11 tumors studied, but not in adjacent normal colon. A method was devised for the isolation from colorectal adenocarcinomas of fibroblast-like cells that stained positive for vimentin but negative for cytokeratin. These tumor-derived stromal cells synthesized and secreted vitronectin, as revealed by metabolic labeling and immunoprecipitation. This was confirmed by Southern blot analysis of polymerase chain reaction amplification products from reverse-transcribed RNA. Normal skin fibroblasts did not synthesize vitronectin. Immunofluorescence staining showed vitronectin deposited at focal contact sites in the tumor-derived cells, where it colocalized with vinculin and the alpha v integrin subunit. The deposition of vitronectin into focal contact sites was not dependent on the presence of serum. The finding that vitronectin can be synthesized and secreted by tumor-derived fibroblast-like cells in culture indicates that vitronectin expression can be promoted by as yet unknown signals provided in disease states, such as cancer.

Citations

May 17, 2011·PloS One·Ondrej StepanekVaclav Horejsi
Mar 1, 2012·PloS One·Amandine Cartier-MichaudGeorgia Barlovatz-Meimon
Aug 13, 2011·International Journal of Cell Biology·Ralf-Peter CzekayPaul J Higgins
Jun 18, 2011·Expert Review of Proteomics·Juan Casado-VelaJuan Carlos Lacal
Dec 19, 2003·Cell Communication & Adhesion·Cynthia E Wilkins-PortPaula J McKeown-Longo
Oct 29, 2000·Cell Adhesion and Communication·S A Wilcox-AdelmanP J McKeown-Longo
Aug 6, 2015·Journal of Cancer Research and Clinical Oncology·Michael A RogersPeter Lichter
Sep 14, 2013·IUBMB Life·David I LeavesleyZee Upton
Dec 14, 2011·Laboratory Investigation; a Journal of Technical Methods and Pathology·Celia ChaoMark R Hellmich
Jan 1, 1996·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·S A WilcoxP J McKeown-Longo
Sep 10, 1999·The Journal of Biological Chemistry·D C HockingP J McKeown-Longo
Dec 29, 1998·The Journal of Biological Chemistry·Q ZhangD F Mosher
May 9, 2002·Biochimica Et Biophysica Acta·Seiko ShimizuMasao Hayashi
Jan 5, 2002·Human Pathology·G K KoukoulisV E Gould

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.