Vm-milR37 contributes to pathogenicity by regulating glutathione peroxidase gene VmGP in Valsa mali.

Molecular Plant Pathology
Hao FengLili Huang

Abstract

MicroRNAs play important roles in various biological processes by regulating their corresponding target genes. However, the function and regulatory mechanism of fungal microRNA-like RNAs (milRNAs) are still largely unknown. In this study, a milRNA (Vm-milR37) was isolated and identified from Valsa mali, which causes the most serious disease on the trunk of apple trees in China. Based on the results of deep sequencing and quantitative reverse transcription PCR, Vm-milR37 was found to be expressed in the mycelium, while it was not expressed during the V. mali infection process. Overexpression of Vm-milR37 did not affect vegetative growth, but significantly decreased pathogenicity. Based on degradome sequencing, the target of Vm-milR37 was identified as VmGP, a glutathione peroxidase. The expression of Vm-milR37 and VmGP showed a divergent trend in V. mali-apple interaction samples and Vm-milR37 overexpression transformants. The expression of VmGP could be suppressed significantly by Vm-milR37 when coexpressed in tobacco leaves. Deletion of VmGP showed significantly reduced pathogenicity compared with the wild type. VmGP deletion mutants showed more sensitivity to hydrogen peroxide. Apple leaves inoculated with Vm-milR37 overexpre...Continue Reading

References

Jul 18, 2002·The Plant Cell·Cesar LlaveJames C Carrington
Oct 7, 2004·Fungal Genetics and Biology : FG & B·Jae-Hyuk YuClaudio Scazzocchio
Dec 14, 2004·Eukaryotic Cell·Kenneth S BrunoJin-Rong Xu
Jun 21, 2006·Journal of Molecular Evolution·Hitoshi NakayashikiShigeyuki Mayama
Jun 11, 2008·Nature Biotechnology·Marcelo A GermanPamela J Green
Jan 17, 2009·Nature Reviews. Genetics·Megha Ghildiyal, Phillip D Zamore
Feb 26, 2009·Cell·Richard W Carthew, Erik J Sontheimer
Jan 10, 2008·Annual Review of Cell and Developmental Biology·Travis Thomson, Haifan Lin
Oct 14, 2009·Proceedings of the National Academy of Sciences of the United States of America·Qihong SunDonald L Nuss
Aug 7, 2010·Annual Review of Phytopathology·Surekha Katiyar-Agarwal, Hailing Jin
Nov 19, 2011·Sub-cellular Biochemistry·May T Aung-HtutIan W Dawes
Dec 14, 2011·Plant Physiology and Biochemistry : PPB·Hao FengZhensheng Kang
Jul 4, 2012·Annual Review of Microbiology·Shwu-Shin ChangYi Liu
Mar 20, 2013·World Journal of Microbiology & Biotechnology·Zhiyuan YinLili Huang
Apr 22, 2014·Fungal Genetics and Biology : FG & B·Xiwang KeLili Huang
May 17, 2014·Ecology and Evolution·Xuli WangLili Huang
Jan 3, 2015·Nature Reviews. Genetics·Daniel Holoch, Danesh Moazed
Sep 24, 2015·BMC Genomics·Nicholas A MuethScot H Hulbert
Jul 5, 2016·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Runmao LinAiping Zheng
Aug 30, 2016·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Azali AzlanGhows Azzam
Apr 21, 2017·PLoS Pathogens·Kar-Chun Tan, Richard P Oliver

❮ Previous
Next ❯

Methods Mentioned

BETA
degradome sequencing
reverse transcription PCR
PCR
confocal microscopy
transfection

Software Mentioned

BLAST
WoLF PSORT
CLUSTAL X2
MEGA
ImageJ
BlastP

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.