Vocal fold myofibroblast profile of scarring

The Laryngoscope
Anete BrancoSusan L Thibeault

Abstract

Vocal fold fibroblasts (VFF) are responsible for extracellular matrix synthesis supporting lamina propria in normal and diseased conditions. When tissue is injured, VFF become activated and differentiate into myofibroblasts to facilitate wound healing response. We investigated if vocal fold myofibroblasts can be utilized as surrogate cells for scarred VFF. In vitro. Normal VFF cell lines from a 21-year-old male (N21), 59-year-old female (N59), and a scar VFF cell line from a 56-year-old female (S56) were used in this study. 10 ng/mL of transforming growth factor (TGFβ1) was applied for 5 days to normal VFF. Myofibroblast differentiation was determined with immunocytochemistry and western blot, measuring alpha smooth muscle actin (α-SMA). Cell growth, proliferation, contractile properties, and gene expression profiles were evaluated. N21, N59, and S56 VFF presented elongated configuration. N21+ and N21- VFF demonstrated significantly greater proliferation compared to N59+, N59-, and S56 VFF at 6 days. α-SMA was expressed in all cells. Fibronectin, alpha smooth actin, connective tissue growth factor, and metallopeptidase inhibitor were the highest genes expression in VFF treated with transforming growth factor β1 (TGFβ1). At 24 h...Continue Reading

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Citations

Sep 6, 2018·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Hongyu ChenMariah S Hahn
Mar 10, 2018·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·M GrauppM Gugatschka
Apr 25, 2019·BMC Genomics·Alexander G FooteSusan L Thibeault
Apr 16, 2020·The Laryngoscope·Haoyuan Xu, Guo-Kang Fan
Nov 20, 2016·The Laryngoscope·Tanaya WalimbeM Preeti Sivasankar
Jun 6, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Josh D Erndt-MarinoMariah S Hahn
Jan 16, 2021·Tissue Engineering. Part B, Reviews·Ana M Gracioso MartinsDonald O Freytes
Jul 1, 2021·Scientific Reports·Ryosuke NakamuraRyan C Branski

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