Volatile Oil of Acori Graminei Rhizoma-Induced Apoptosis and Autophagy are dependent on p53 Status in Human Glioma Cells

Scientific Reports
Lu ChenHongyi Qi

Abstract

Acori Graminei Rhizoma is well known for the beneficial effects on CNS disorders in traditional medicine. Though it is frequently prescribed in formulations for brain tumors, the anti-glioma effect has not been examined. We used volatile oil of Acori Graminei Rhizoma (VOA) and human glioblastoma multiforme (GBM) cells in this study. We found that VOA exhibited greater growth suppression in p53 wild-type cells than p53 mutant cells and very low effect on fibroblasts and human glial HEB cells. Apoptosis was triggered by VOA with a caspase-dependent way in p53 wild-type A172 cells, while a caspase-independent way in p53 mutant U251 cells. Meanwhile, both A172 and U251 cells treated by VOA displayed autophagic features. Furthermore, p53 decrease was observed along with VOA-induced apoptosis and autophagy in A172 cells. VOA-induced autophagy was mediated through a p53/AMPK/mTOR signaling pathway in A172 cells, while an mTOR-independent signaling pathway in U251 cells. Finally, blockage of autophagy potentiated the proapoptotic effect in both A172 and U251 cells, indicating a protective role of autophagy in VOA-induced cell death. Together, VOA exhibited anti-tumor activity in human GBM cells and induced apoptotic cell death and prot...Continue Reading

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Citations

Mar 17, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Li LiXiaoyu Xu
May 13, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Li LiXiaoyu Xu
Nov 16, 2018·Apoptosis : an International Journal on Programmed Cell Death·Shijie CaoNing Kang
Sep 6, 2017·Asian Pacific Journal of Tropical Medicine·Bimal Kumar GhimireIll-Min Chung
Nov 29, 2017·BioMed Research International·Moon Nyeo ParkBonglee Kim

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Methods Mentioned

BETA
flow cytometry
transfection
electrophoresis

Software Mentioned

Flow Jo

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