Volatile organic compounds discriminate between eosinophilic and neutrophilic inflammation in vitro

Journal of Breath Research
Florence N SchleichF J Van Schooten

Abstract

Inflammation associated oxidative stress leads to peroxidation of polyunsaturated fatty acids thereby generating volatile organic compounds (VOCs). The integrative analysis of the total amount of VOCs released by eosinophils and neutrophils in vitro enables the search for those compounds that discriminates between various inflammatory conditions. The approach comprises isolating eosinophils and neutrophils from 30 ml of blood of healthy non-smoking volunteers by gradient centrifugation, using lymphoprep. Eosinophils are separated from neutrophils by immunomagnetic cell separation using anti-CD16. Cells are activated with phorbol 12-myristate 13-acetate and VOCs from the headspace are collected at time 0', 30', 60' and 90' by introduction of ultra-pure nitrogen in the closed flasks at a flow rate of 200 ml min(-1) during 10 min. The gases are trapped onto a sorption tube and analyzed by gas chromatography-time-of-flight-mass spectometry (GC-TOF-MS) in order to identify VOCs released in the headspace by activated neutrophils and eosinophils. Eosinophils and neutrophils were isolated from 26 healthy non-smoking volunteers. The average absolute number of eosinophils and neutrophils upon isolation was 3.5  ×  10(6) and 19.4  ×  10(6...Continue Reading

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Citations

Jun 20, 2017·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P BrinkmanN Fens
Oct 17, 2018·Current Opinion in Pulmonary Medicine·Paul BrinkmanAriane H Wagener
Jan 13, 2018·The European Respiratory Journal·Stephen J Fowler
Apr 12, 2019·American Journal of Respiratory and Critical Care Medicine·Florence N SchleichRenaud Louis
Oct 4, 2017·Journal of Breath Research·R R R FijtenF J van Schooten
Jun 9, 2020·Journal of Breath Research·Laura TeneroGiorgio L Piacentini
Jul 6, 2019·The European Respiratory Journal·Adnan AzimPeter Howarth
Oct 2, 2020·Scientific Reports·Pierre-Hugues StefanutoJean-François Focant
Oct 1, 2019·The European Respiratory Journal·Stephen J Fowler, Peter J Sterk
May 1, 2019·Applied in Vitro Toxicology·Brett R WintersMichael C Madden
Feb 29, 2020·The European Respiratory Journal·Florence N SchleichJean-François Focant
Nov 24, 2020·Journal of Breath Research·Rosa PeltriniSalman Siddiqui
Feb 4, 2021·Journal of Asthma and Allergy·Sophie GraffFlorence Schleich

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