Volatile products from acetylcholine as markers in the rapid urine test using head-space gas-liquid chromatography

Journal of Chromatography
T Davies, N J Hayward

Abstract

Head-space gas--liquid chromatographic analyses of cultures of all Proteus spp. showed that the production of trimethylamine from acetylcholine could be the marker for the detection in 3.5 h of significant numbers of P. mirabilis, P. vulgaris, P. rettgeri and P. inconstans A in the rapid test for Escherichia and Klebsiella spp. in urine specimens. Trimethylamine was not detected in cultures of five other urinary pathogens. Six of fifteen strains of K. aerogenes produced trimethylamine from acetylcholine but were distinguished from Proteus spp. by ethanol production from arabinose. Ethyl acetate was produced from acetylcholine by P. mirabilis, P. vulgaris, P. rettgeri, P. inconstans A, P. inconstans B, E. coli, K. aerogenes and Streptococcus faecalis.

Citations

Feb 4, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·A Marzo, S Curti
May 16, 2013·PLoS Pathogens·Lieuwe D J BosMarcus J Schultz
Feb 22, 2012·Sensors·Alphus D Wilson, Manuela Baietto
Jul 5, 2013·Clinical Microbiology Reviews·Shneh SethiTrinad Chakraborty
May 24, 2012·Expert Review of Molecular Diagnostics·Ashish GuptaBrian Houston
Jul 29, 2020·Biosensors·Valentin-Mihai DospinescuJames A Covington

Related Concepts

Ethyl acetate
HCl of trimethylamine
Acetic Acids
Acetilcolina Cusi
Ethanol
Arabinose
Bacteriuria
Chromatography, Gas-Liquid
Alkalescens-Dispar Group
Klebsiella rhinoscleromatis

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