Voltage-activated ionic currents in differentiating rat cerebellar granule neurons cultured from the external germinal layer

Journal of Neurobiology
R R StewartA Feltz

Abstract

The electrical properties of the precursor cells of the external germinal layer of rat cerebellum were assessed during their differentiation in control medium (Dulbecco's modified Eagle's medium) supplemented or not with either basic fibroblast growth factor (bFGF) or 25 mM potassium chloride (KCl). Resting potential was shown to be -10 mV in all three conditions 3 hours after plating [days in vitro (DIV)0]. By DIV 5, it reached -63 mV for cells cultured in 25 mM KCl but only -28 mV in control and bFGF media. The main voltage-sensitive ionic current measured at DIV 0 under all conditions was a composite IK consisting in a sustained K+ current blocked by tetraethylammonium (IK(TEA)), plus a rapidly activating and inactivating TEA-insensitive IK(A). Both currents increased with time in all conditions, but after 5 days IK(A) became dominant in terms of density. IK(TEA) is likely an IK(Ca), since it was blocked by 67% in 1 mM TEA. On DIV 0, INa and ICa were absent or small in amplitude. By DIV 3, 80% of the cells had currents able to generate a spike. Interestingly, ICa mean amplitude and current density measured at -10 mV in control condition on DIV 1 was significantly larger than those recorded in bFGF and 25 mM KCl. The order of...Continue Reading

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Citations

Sep 23, 2003·Experimental Cell Research·Virginie GeeraertJean de Barry
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y HumeauB Poulain
Aug 25, 2007·Molecular and Cellular Neurosciences·Hervé AptelFrédérique Scamps
Aug 16, 2016·Cellular and Molecular Neurobiology·Katarína OndáčováĽubica Lacinová
Jul 18, 2003·Journal of Neuroscience Research·Cristina HervásMa Teresa Miras-Portugal
Mar 9, 2018·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Amynah A PradhanSimon Akerman
Jan 1, 1997·Journal of Neurobiology·U BechererA Feltz

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