Voltage clamp analysis of acetylcholine produced end-plate current fluctuations at frog neuromuscular junction

The Journal of Physiology
C R Anderson, C F Stevens

Abstract

1. Acetylcholine produced end-plate current (e.p.c.) noise is shown to be the results of statistical fluctuations in the ionic conductance of voltage clamped end-plates of Rana pipiens.2. These e.p.c. fluctuations are characterized by their e.p.c. spectra which conform to a relation predicted from a simple model of end-plate channel gating behaviour.3. The rate constant of channel closing alpha is determined from e.p.c. spectra and is found to depend on membrane potential V according to the relation alpha = Be(AV) (B = 0.17 msec(-1)+/-0.04 S.E., A = 0.0058 mV(-1)+/-0.0009 S.E. at 8 degrees C) and to vary with temperature T with a Q(10) = 2.77, at -70 mV. A and B in this expression both vary with T and therefore produce a membrane potential dependent Q(10) for alpha.4. Nerve-evoked e.p.c.s and spontaneous miniature e.p.c.s decay exponentially in time with a rate constant which depends exponentially on V. The magnitude and voltage dependence of this decay constant is exactly that found from e.p.c. spectra for the channel closing rate alpha.5. The conductance gamma of a single open end-plate channel has been estimated from e.p.c. spectra and is found not to be detectibly dependent on membrane potential, temperature and mean end-pl...Continue Reading

Citations

Apr 1, 1981·Pflügers Archiv : European journal of physiology·W Zeiske, W Van Driessche
Nov 26, 2010·Pflügers Archiv : European journal of physiology·Claudia MoriconiFrancesca Grassi
Nov 11, 2010·Journal of Computational Neuroscience·Abdul KhaliqWeizhong Dai
Jan 1, 1984·Neir̆ofiziologiia = Neurophysiology·L G MagazanikV A Snetkov
Jun 1, 1978·Neuropharmacology·K KaibaraA G Karczmar
Jun 30, 1976·The Journal of Membrane Biology·L E Moore, E Neher
Dec 4, 1975·The Journal of Membrane Biology·H M FishmanD M Poussart
Jan 1, 1982·The Journal of Membrane Biology·K TakedaP H Barry
Dec 14, 1979·The Journal of Membrane Biology·R N CoxP W Brandt
Aug 21, 1980·The Journal of Membrane Biology·D Marchais, A Marty
Dec 15, 1980·The Journal of Membrane Biology·H Reuter, C F Stevens
May 21, 1979·The Journal of Membrane Biology·L E MooreD J Poussart
Jan 1, 1984·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·H van WilgenburgP Mantel
Jan 1, 1981·Comparative Biochemistry and Physiology. C: Comparative Pharmacology·J E Chad, G A Kerkut
Jan 1, 1995·European Biophysics Journal : EBJ·R A GiniatullinF Vyskocil
Jan 1, 1984·European Biophysics Journal : EBJ·P Läuger
Jul 1, 1992·Pflügers Archiv : European journal of physiology·R Schneggenburger, A Konnerth
Apr 1, 1995·Progress in Neurobiology·T Akasu, T Nishimura
Jan 1, 1987·Progress in Neurobiology·M L Mayer, G L Westbrook
Jan 1, 1984·Progress in Neurobiology·K S Rózsa
Jan 1, 1978·Progress in Neurobiology·E M Landau
Jan 1, 1976·Progress in Neurobiology·H L Atwood
Jan 1, 1976·Progress in Neurobiology·A R Freeman
Mar 1, 1994·Progress in Neurobiology·K Kaila
Jul 1, 1976·Neuroscience and Behavioral Physiology·V V Fedorov
Dec 1, 1991·Journal of Neurocytology·P R Lyons, C R Slater
Jan 1, 1986·Progress in Biophysics and Molecular Biology·F Ramón, A Rivera
Jan 1, 1985·Progress in Biophysics and Molecular Biology·H M Fishman
Jan 1, 1974·Progress in Biophysics and Molecular Biology·A A Verveen, L J DeFelice
Dec 29, 1977·Molecular and Cellular Biochemistry·F Hucho, W Schiebler
Dec 1, 1987·Journal of the Autonomic Nervous System·V I Skok
Jul 1, 1982·Journal of the Autonomic Nervous System·K KoketsuK Hirai
Dec 1, 1979·Journal of the Autonomic Nervous System·A A SelyankoV I Skok
Jan 1, 1997·Biosensors & Bioelectronics·Y Y YuW C Davis
Sep 1, 1993·Journal of Pharmacological and Toxicological Methods·C PriorI G Marshall

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