PMID: 3746395Jul 1, 1986Paper

Voltage-clamp analysis of currents produced by glutamate and some glutamate analogues on horizontal cells isolated from the catfish retina

Journal of Neurophysiology
G HalsR Shingai

Abstract

Horizontal cells isolated from the catfish retina were exposed to radiolabeled glutamate, glycine, gamma-aminobutyric acid (GABA), and sucrose to determine if the enzymatic dissociation procedure altered the high-affinity uptake mechanism for GABA and generally reduced membrane selectivity. As in the intact retina, isolated cells could transport GABA but not the other substances. The horizontal cells were voltage clamped using a single low-resistance patch-type electrode. The acidic amino acid L-glutamate, and its analogues kainate and quisqualate, were applied to the cell by pressure ejection from a nearby pipette. All three agonists produced inward currents that reversed near O mV. Quisqualate produced a current with a similar time course as glutamate, but the time course of the response to kainate was faster. The agonists N-methyl-D-aspartate and L-aspartate had little effect on the membrane conductance. The current-to-voltage (I-V) relationship for all three agonists was nonlinear when the membrane potential was hyperpolarized. The nonlinearity was, at least in part, a result of the decreased response to the three agonists. Removal of Mg did not alter this nonlinear relationship. When the inward potassium rectifier was bloc...Continue Reading

Citations

Apr 5, 2003·American Journal of Physiology. Cell Physiology·Scott F Davis, Cindy L Linn
Nov 29, 2002·American Journal of Physiology. Cell Physiology·S F Davis, C L Linn
Jan 1, 1997·Visual Neuroscience·S Eliasof, C E Jahr
May 13, 1996·Brain Research·M A Micci, B N Christensen

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