Voltage clamp limitations of dual whole-cell gap junction current and voltage recordings. I. Conductance measurements

Biophysical Journal
R D Veenstra

Abstract

Previous correction methods for series access resistance errors in the dual whole-cell configuration did not take into account the effect of nonzero resting potentials (E(rest)) and junctional reversal potentials (E(rev)). Dual whole-cell currents were modeled according to resistor-circuit analysis and two correction formulas for the measurement of junctional currents (I(j)) were assessed. The equations for I(j), derived from Kirchoff's law before and after baseline subtraction of the nonjunctional current, were assessed for accuracy under a variety of whole-cell patch-clamp recording conditions. Both equations accurately correct for dual whole-cell voltage-clamp errors provided that the cellular parameters are included in the nonbaseline subtracted I(j) derivations. Junctional conductance (g(j)) estimates are most reliable at high junctional resistance (R(j)) values and minimize the need for corrective methods based on electrode series and cellular input resistances (R(el) and R(in)). In the "open-cell" configuration, low R(j) values relative to R(in) are required for accurate g(j) estimates. These methods provide the basis for accurate quantitative measurements of junctional resistance (or conductance) of gap junction channel...Continue Reading

References

Jul 1, 1992·Biophysical Journal·H Z WangR D Veenstra
Feb 1, 1991·The Journal of Membrane Biology·R D Veenstra
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L C BarrioB J Nicholson
Sep 2, 1985·Nature·J Neyton, A Trautmann
Aug 29, 1986·Science·R D Veenstra, R L DeHaan
Jun 1, 1989·Journal of Protein Chemistry·R Somogyi, H A Kolb
Feb 1, 1985·Circulation Research·F MazetD C Spray
Jan 1, 1981·The Journal of General Physiology·D C SprayM V Bennett
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Oct 1, 1984·The Journal of Physiology·J De BruijneA C van Ginneken
Apr 1, 1995·Pflügers Archiv : European journal of physiology·F F BukauskasR Weingart
Mar 24, 1994·Nature·V K VerselisT A Bargiello
Nov 1, 1993·The Journal of General Physiology·M ChansonD C Spray
Aug 1, 1996·Journal of Bioenergetics and Biomembranes·R D Veenstra
May 1, 1997·Biophysical Journal·X W ZhouG Dahl
Jun 20, 1998·Pflügers Archiv : European journal of physiology·H V Van RijenH J Jongsma

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Citations

Nov 12, 2005·The Journal of Membrane Biology·S V RamananP R Brink
Apr 24, 2008·Cardiovascular Research·Xianming LinRichard D Veenstra
Mar 17, 2012·American Journal of Physiology. Cell Physiology·Qin XuRichard D Veenstra
Oct 30, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Xianming LinRichard D Veenstra
Jun 3, 2009·Journal of Molecular and Cellular Cardiology·Xianming LinRichard D Veenstra
May 1, 2007·Biophysical Journal·Xianming Lin, Richard D Veenstra
Feb 23, 2008·Biochemical and Biophysical Research Communications·Joanna GemelEric C Beyer
May 28, 2003·Biochemical and Biophysical Research Communications·Martina BeltramelloPaola D'Andrea
Jul 24, 2008·Cell Communication & Adhesion·Jan Sebastian SchulteStefan Dhein
Jun 29, 2014·Journal of Molecular and Cellular Cardiology·Joanna GemelEric C Beyer
Jan 14, 2003·Biophysical Journal·Hassan Musa, Richard D Veenstra
Dec 2, 2005·Journal of Theoretical Biology·Pierre A Fortier, Maimouna Bagna
Nov 15, 2005·The Journal of Physiology·Xianming LinRichard D Veenstra
Aug 30, 2003·Circulation Research·Xianming LinRichard D Veenstra
Jan 18, 2014·FEBS Letters·Dakshesh PatelRichard D Veenstra
Feb 25, 2005·Journal of Neurophysiology·Pascale RabbahFarzan Nadim
Dec 31, 2004·American Journal of Physiology. Endocrinology and Metabolism·Alonso P MorenoE Martha Pérez-Armendariz
Jan 31, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mark A StaggCesare M N Terracciano
Nov 3, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Qin XuRichard D Veenstra
Jul 18, 2002·The Journal of Biological Chemistry·Gary S GoldbergPaul D Lampe
Dec 25, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Xianming Lin, Richard D Veenstra

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