PMID: 9556347Apr 7, 1998Paper

Voltage clamp studies on S-layer-supported tetraether lipid membranes

Biochimica Et Biophysica Acta
B SchusterU B Sleytr

Abstract

Isolated subunits from the cell surface proteins (S-layer) of Bacillus coagulans E38-66 have been recrystallized on a glycerol dialkyl nonitol tetraether lipid (GDNT)-monolayer and the electrophysical features of this biomimetic membrane have been investigated in comparison to unsupported GDNT-monolayers. The GDNT-monolayer, spread on a Langmuir-Blodgett trough, was clamped with the tip of a glass patch pipette. In order to investigate the barrier function and potential to incorporate functional molecules, voltage-clamp examinations on plain and S-layer-supported GDNT-monolayers were per-formed. Our results indicate the formation of a tight GDNT-monolayer sealing the tip of the glass pipette, and a decrease in conductance of the GDNT-monolayer upon recrystallization of the S-layer protein. Thus, the S-layer protein, apparently, did not penetrate or rupture the lipid monolayer. The valinomycin-mediated increase in conductance was less pronounced for the S-layer-supported than for the plain GDNT-monolayer, indicating differences in the accessibility and/or in the fluidity of the lipid membranes. Furthermore. in contrast to plain GDNT-monolayers. S-layer supported GDNT-monolayers with high valinomycin-mediated conductance persiste...Continue Reading

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Citations

Feb 27, 1999·Current Opinion in Biotechnology·H Bayley
Jan 6, 2001·Journal of Biotechnology·B Schuster, U B Sleytr
Jun 1, 2008·Biointerphases·Bernhard SchusterUwe B Sleytr
May 9, 2014·Journal of the Royal Society, Interface·Bernhard Schuster, Uwe B Sleytr
Jan 12, 2010·Chemistry and Physics of Lipids·Parkson Lee-Gau Chong
Mar 24, 2009·Journal of Structural Biology·Bernhard Schuster, Uwe B Sleytr
May 21, 2009·Biochimie·Alicia JacquemetThierry Benvegnu
Jul 9, 2003·IEEE Engineering in Medicine and Biology Magazine : the Quarterly Magazine of the Engineering in Medicine & Biology Society·U B SleytrD Pum
Apr 21, 1999·Biochimica Et Biophysica Acta·C MaderU B Sleytr
Feb 4, 2014·FEMS Microbiology Reviews·Uwe B SleytrDietmar Pum
Mar 1, 2007·FEMS Microbiology Letters·Uwe B SleytrEva M Egelseer
Sep 24, 2004·IEEE Transactions on Nanobioscience·Bernhard SchusterUwe B Sleytr
Apr 5, 2017·Advances in Colloid and Interface Science·Élodie BoisselierChristian Salesse
Sep 25, 2007·Journal of Structural Biology·Harald Engelhardt
Apr 12, 2018·Biosensors·Bernhard Schuster
Feb 16, 2006·IEE Proceedings. Nanobiotechnology·D PumU B Sleytr
Mar 25, 2020·Sensors·Samar Damiati, Bernhard Schuster
May 16, 1998·Biochimica Et Biophysica Acta·B SchusterU B Sleytr

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