PMID: 7517034Mar 1, 1994Paper

Voltage clamping of Xenopus laevis oocytes utilizing agarose-cushion electrodes

Pflügers Archiv : European journal of physiology
Wolfgang SchreibmayerNathan Dascal

Abstract

Two-electrode voltage clamping of expressed ion channels in intact oocytes of the South African clawed frog Xenopus laevis has been refined to allow stable, low-resistance electrical access to the cytosol (50-800 k omega). Glass microelectrodes were filled with a cushion of 1% agarose at their tips to prevent KCl leakage (agarose-cushion electrodes). Insertion of these electrodes into X. laevis oocytes yielded stable preparations for periods of more than 1 h with a stable input resistance of 1-4 M omega. Furthermore, a simple modification of the voltage-clamp circuit (charging compensator) is described that increases the flexibility of arrangements for differential recording of the membrane potential in order to subtract voltage drops across a series resistance. The result is a considerable increase in the practically attainable speed of the voltage clamp with the conventional two-electrode arrangement. The performance of the charging compensator was tested on an equivalent circuit that simulates the oocyte and electrodes. In addition, the combination of agarose-cushion electrodes and the charging compensator was tested on oocytes expressing Shaker H4 currents. The fidelity of the voltage-clamp circuit was also verified by meas...Continue Reading

References

Jan 1, 1987·CRC Critical Reviews in Biochemistry·N Dascal
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L E IversonB Rudy
Oct 1, 1980·The Journal of Physiology·F J Sigworth
Dec 1, 1980·Journal of Neuroscience Methods·T G SmithT R Colburn
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·N DascalH A Lester
Apr 1, 1952·The Journal of Physiology·A L HODGKINB KATZ

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Citations

Jan 1, 1996·Pflügers Archiv : European journal of physiology·L DiMagnoW Schreibmayer
Aug 7, 2007·The Journal of Membrane Biology·Xian-Sheng LiuGea-Ny Tseng
Jan 20, 2009·Pflügers Archiv : European journal of physiology·Radda RusinovaDiomedes E Logothetis
May 16, 1997·Journal of Neuroscience Methods·P C RubenM D Rayner
Aug 4, 2012·Chemical Research in Toxicology·Michael J TwinerMichael C Sanguinetti
Nov 4, 2010·The Journal of Biological Chemistry·Amanda M StyerTooraj Mirshahi
Apr 18, 2009·The Journal of Biological Chemistry·Min JiangGea-Ny Tseng
Jun 1, 1997·The Journal of General Physiology·C C ShiehG E Kirsch
Oct 31, 2002·The Journal of General Physiology·Jie LiuGea-Ny Tseng
Nov 17, 2004·The Journal of General Physiology·Mei ZhangGea-Ny Tseng
Aug 30, 2012·The Journal of General Physiology·Vivek GargMichael C Sanguinetti
Nov 28, 2012·The Journal of General Physiology·Yuhong WangGea-Ny Tseng
Oct 30, 2013·The Journal of General Physiology·Priyanka GargMichael C Sanguinetti
Aug 2, 2012·Molecular Pharmacology·Priyanka Garg, Michael C Sanguinetti
Dec 20, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Mei ZhangGea-Ny Tseng
Mar 19, 2014·The Journal of General Physiology·Wei WuMichael C Sanguinetti
Jan 15, 2014·The Journal of Physiological Sciences : JPS·Yu Kodani, Yasuo Furukawa
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·P P DuquetteJ Y Lapointe
Dec 22, 2007·Journal of Receptor and Signal Transduction Research·Bibiane SteineckerWolfgang Schreibmayer

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