Voltage dependence of transepithelial guanidine permeation across Caco-2 epithelia allows determination of the paracellular flux component

Pharmaceutical Research
Georgina CarrNicholas L Simmons

Abstract

The aim of this study was to investigate transepithelial ionic permeation via the paracellular pathway of human Caco-2 epithelial monolayers and its contribution to absorption of the base guanidine. Confluent monolayers of Caco-2 epithelial cells were mounted in Ussing chambers and the transepithelial conductance and electrical potential difference (p.d.) determined after NaCl dilution or medium Na substitution (bi-ionic conditions). Guanidine absorption (Ja-b) was measured +/- transepithelial potential gradients using bi-ionic p.d.'s. Basal NaCl replacement with mannitol gives a transepithelial dilution p.d. of 28.0 +/- 3.1 mV basal solution electropositive (PCl/PNa = 0.34). Bi-ionic p.d.'s (basal replacements) indicate a cation selectivity of NH4+ > K+ approximately Cs+ > Na+ > Li+ > tetraethylammonium+ > N-methyl-D: -glucamine+ approximately choline+. Transepithelial conductances show good correspondence with bi-ionic potential data. Guanidine Ja-b was markedly sensitive to imposed transepithelial potential difference. The ratio of guanidine to mannitol permeability (measured simultaneously) increased from 3.6 in the absence of an imposed p.d. to 13.8 (basolateral negative p.d.). Hydrated monovalent ions preferentially perme...Continue Reading

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Citations

Nov 9, 2010·The Journal of Membrane Biology·Georgina CarrNicholas L Simmons
Apr 8, 2011·World Journal of Gastroenterology : WJG·Narongrit Thongon, Nateetip Krishnamra
Jul 17, 2007·Journal of Pharmaceutical Sciences·Ana Ruiz-GarciaVicente G Casabo
May 10, 2007·American Journal of Physiology. Endocrinology and Metabolism·Walailuk JantarajitNarattaphol Charoenphandhu
Jul 2, 2009·American Journal of Physiology. Endocrinology and Metabolism·Narattaphol CharoenphandhuNateetip Krishnamra
Mar 21, 2008·American Journal of Physiology. Cell Physiology·Narongrit ThongonNarattaphol Charoenphandhu

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