PMID: 9430818Feb 7, 1998Paper

Voltage dependent calcium and potassium currents in Y-1 adrenocortical cells are unresponsive to ACTH

Endocrine Research
M D CoyneJ R Lemos

Abstract

In this report we use both whole cell and perforated patch clamp recording techniques to characterize calcium and potassium channels in Y-1 adrenocortical cells in order to assess their responsiveness to ACTH. Both transient and long-lasting components of an inward calcium current were identified which were similar to T and L-type Ca2+ currents. With Ba2+ as the charge carrier, the transient current activated at voltages more hyperpolarized than -50 mV with V1/2 for activation at -78.1 mV, and for steady state inactivation at -52.3 mV. The L-type current activated at -20 mV, with a V1/2 for activation at -29.9 mV and steady state inactivation at -44.2 mV. Under perforated patch conditions the response was shifted to more depolarized voltages. Both currents were responsive to agents which usually affect T- or L-type Ca2+ currents. The transient current was completely blocked by 50 microM lanthanum or 200 microM nickel and partially blocked by 300 mM amiloride. Cadmium (100 microM) and nifedipine (300 nM) completely blocked the long-lasting current while omega-conotoxin GVIA (1992 nM) inhibited the current by only 20-25%. The agonist, Bay K 8644 was stimulatory at 50 nM. Both transient and sustained outward potassium currents sim...Continue Reading

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Citations

Jun 16, 2004·The International Journal of Neuroscience·Waleed B Alshuaib, Mini V Mathew
Jan 15, 1999·Endocrine Research·M EsneuM D Payet
Jun 10, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·C S KimJ K Gwathmey

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