PMID: 9651467Jul 4, 1998Paper

Voltage-dependent calcium influx in human sperm assessed by simultaneous optical detection of intracellular calcium and membrane potential

Biochimica Et Biophysica Acta
L Linares-HernándezMarco T González-Martínez

Abstract

There are several physiological and pharmacological evidences indicating that opening of voltage dependent calcium channels play a crucial role in the induction of the acrosome reaction in mammalian sperm. In mature sperm, physiological inductors of the acrosome reaction such as ZP3, a zona pellucida protein, and the steroid hormone progesterone, induce depolarization and calcium influx, which are required for the acrosome reaction. In this paper, we describe a voltage-dependent calcium influx present in human sperm. We report an experimental procedure that allows measurement of intracellular calcium and membrane potential simultaneously using the fluorescent dyes DiSC3(5) and Fura-2. We found that in human uncapacitated sperm, depolarization induces a nifedipine-insensitive calcium influx that, in most cases, was transient. Calcium influx was observed in the range of -60 to -15 mV (the range tested). At resting membrane potential (around -40 mV), potassium addition depolarized and induced calcium influx, but when the depolarization was preceded by a hyperpolarization (induced with valinomycin), calcium influx was remarkably enhanced, suggesting that at -40 mV, channels are in a putative inactivated state. When sperm was incuba...Continue Reading

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Citations

Aug 4, 2011·Human Reproduction·Víctor Torres-FloresMarco T González-Martínez
Jun 7, 2011·Molecular Human Reproduction·Yuriy Kirichok, Polina V Lishko
Oct 25, 2011·Annual Review of Physiology·Polina V LishkoDavid E Clapham
May 29, 2000·Journal of Cardiovascular Electrophysiology·V G Fast, R E Ideker
Oct 16, 2012·Fertility and Sterility·Woo-Sung KwonMyung-Geol Pang
Aug 4, 2010·The Journal of Physiology·Polina V Lishko, Yuriy Kirichok
Mar 15, 2006·Biochemical and Biophysical Research Communications·Paloma del Carmen Neri-VidaurriMarco T González-Martínez
Dec 18, 2013·Systems Biology in Reproductive Medicine·Jorge Parodi
Dec 5, 2002·Archives of Biochemistry and Biophysics·Marco T González-MartínezAlberto Martín Guzmán-Grenfell
Apr 17, 2014·Molecular Human Reproduction·I López-GonzálezC L Treviño
May 23, 2001·Biology of Reproduction·A RevelliD Ghigo
Jul 31, 2001·Journal of Biomechanical Engineering·D M Geddes, R S Cargill
Aug 13, 2004·American Journal of Physiology. Cell Physiology·Juan J Fraire-Zamora, Marco T González-Martínez
May 4, 2001·Molecular Reproduction and Development·E TostiG Gragnaniello
Nov 4, 2006·The EMBO Journal·Naoya OnoharaHitoshi Kurose
Aug 31, 2002·Endocrine Reviews·Alberto RevelliIlan Tur-Kaspa
Feb 23, 2010·Systems Biology in Reproductive Medicine·Jorge ParodiFernando Romero Mejías
Oct 21, 2011·Physiological Reviews·Alberto DarszonClaudia L Treviño
Oct 31, 2019·Human Reproduction Update·Sean G BrownSarah J Martins da Silva
Apr 30, 1999·Physiological Reviews·A DarszonF Espinosa
Aug 15, 2018·Frontiers in Cell and Developmental Biology·Lis C Puga MolinaMariano G Buffone
Jun 28, 2019·Frontiers in Cell and Developmental Biology·Carolina Baro GrafDarío Krapf
Oct 3, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Francisco Jiménez-TrejoOfir Picazo
Jan 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A HashimotoM Iino
Feb 6, 2020·Frontiers in Cell and Developmental Biology·Carolina Baro GrafDario Krapf
Dec 31, 2020·International Journal of Molecular Sciences·Arturo Matamoros-VolanteClaudia L Treviño
Jul 16, 2003·The Journal of Biological Chemistry·Marco T González-Martínez
Nov 7, 2000·Biochimica Et Biophysica Acta·F M Flesch, B M Gadella

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