Voltage-dependent gating and gating charge measurements in the Kv1.2 potassium channel

The Journal of General Physiology
Itzel G IshidaLeón D Islas

Abstract

Much has been learned about the voltage sensors of ion channels since the x-ray structure of the mammalian voltage-gated potassium channel Kv1.2 was published in 2005. High resolution structural data of a Kv channel enabled the structural interpretation of numerous electrophysiological findings collected in various ion channels, most notably Shaker, and permitted the development of meticulous computational simulations of the activation mechanism. The fundamental premise for the structural interpretation of functional measurements from Shaker is that this channel and Kv1.2 have the same characteristics, such that correlation of data from both channels would be a trivial task. We tested these assumptions by measuring Kv1.2 voltage-dependent gating and charge per channel. We found that the Kv1.2 gating charge is near 10 elementary charges (eo), ∼25% less than the well-established 13-14 eo in Shaker. Next, we neutralized positive residues in the Kv1.2 S4 transmembrane segment to investigate the cause of the reduction of the gating charge and found that, whereas replacing R1 with glutamine decreased voltage sensitivity to ∼50% of the wild-type channel value, mutation of the subsequent arginines had a much smaller effect. These data ...Continue Reading

References

Apr 29, 1975·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·R H Adrian
Jan 1, 1978·Reviews of Physiology, Biochemistry and Pharmacology·W Almers
Jan 1, 1992·Methods in Enzymology·S H Heinemann, F Conti
Oct 1, 1980·The Journal of Physiology·F J Sigworth
Feb 1, 1994·Quarterly Reviews of Biophysics·F J Sigworth
Jun 1, 1994·Biophysical Journal·P Pérez-Cornejo, T Begenisich
Feb 1, 1994·The Journal of General Physiology·W N ZagottaR W Aldrich
Feb 1, 1994·The Journal of General Physiology·W N ZagottaR W Aldrich
Nov 1, 1993·Biophysical Journal·L Heginbotham, R MacKinnon
Dec 1, 1995·The Journal of General Physiology·B HirschbergJ Patlak
Sep 1, 1996·The Journal of General Physiology·F NocetiE Stefani
Mar 7, 1998·The Journal of General Physiology·N E Schoppa, F J Sigworth
Dec 14, 1999·The Journal of General Physiology·L D Islas, F J Sigworth
Jan 3, 2001·The Journal of General Physiology·L D Islas, F J Sigworth
Aug 9, 2001·Proceedings of the National Academy of Sciences of the United States of America·C GonzalezR Latorre
Nov 22, 2002·Advances in Physiology Education·Osvaldo AlvarezRamon Latorre
Apr 28, 2004·FEBS Letters·Muriel LainéBenoît Roux
May 13, 2004·The Journal of Membrane Biology·A ScholleK Benndorf
Jul 9, 2005·Science·Stephen B LongRoderick Mackinnon
Oct 6, 2005·Neuron·Christopher A Ahern, Richard Horn
May 2, 2007·Proceedings of the National Academy of Sciences of the United States of America·Fabiana V CamposFrancisco Bezanilla
Aug 21, 2007·Biophysical Journal·Vishwanath Jogini, Benoît Roux
Nov 16, 2007·Nature·Abdulrasheed A AlabiKenton J Swartz
Sep 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Carlos A Villalba-GaleaFrancisco Bezanilla
Dec 19, 2008·Nature·Kenton J Swartz
Mar 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Vivian González-PérezDavid Naranjo
Apr 3, 2010·Science·Xiao TaoRoderick MacKinnon
May 21, 2010·Biophysical Journal·Fatemeh Khalili-AraghiKlaus Schulten
Jun 15, 2010·Nature Structural & Molecular Biology·I Scott RamseyDavid E Clapham
Jun 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·Kristen M S O'ConnellMichael M Tamkun
Mar 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Lucie DelemotteWerner Treptow
Jul 27, 2011·The Journal of General Physiology·Meng-chin A LinDiane M Papazian
Dec 14, 2011·The Journal of General Physiology·Sandipan Chowdhury, Baron Chanda
Apr 14, 2012·Science·Morten Ø JensenDavid E Shaw
Jun 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·Daniel SchmidtRoderick MacKinnon

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Citations

Jan 27, 2016·The Journal of General Physiology·Víctor De-la-RosaLeón D Islas
Aug 21, 2015·Biochimica Et Biophysica Acta·Ilsoo Kim, Arieh Warshel
Oct 6, 2016·Biophysical Journal·Ofra Barchad-AvitzurYair Ben-Chaim
Feb 29, 2020·The Journal of General Physiology·Esteban Suárez-DelgadoLeón D Islas
Mar 24, 2018·The Journal of Chemical Physics·Agata Wawrzkiewicz-Jałowiecka, Zbigniew J Grzywna
Jul 19, 2019·Proceedings of the National Academy of Sciences of the United States of America·Leo C T NgPaul G DeCaen
Aug 24, 2017·Scientific Reports·Victoria A BaronasHarley T Kurata
Jul 25, 2018·Proceedings of the National Academy of Sciences of the United States of America·Ignacio Díaz-FranulicDavid Naranjo
Jan 8, 2019·Biophysical Journal·Tzyy-Leng HorngFrancisco Bezanilla
Jul 16, 2019·Biophysical Journal·Hongbo ChenKatherine A Henzler-Wildman
Aug 24, 2019·The Journal of Physical Chemistry. B·Alisher M Kariev, Michael E Green

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Methods Mentioned

BETA
PCR

Software Mentioned

Vector NTI software package ( Invitrogen )
AlignX
HEKA
WaveMetrics
custom scripts
Pulse and Patchmaster
TASER
Igor Pro

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