Voltage-dependent modulation of single N-Type Ca2+ channel kinetics by receptor agonists in IMR32 cells

Biophysical Journal
V CarabelliE Carbone

Abstract

The voltage-dependent inhibition of single N-type Ca(2+) channels by noradrenaline (NA) and the delta-opioid agonist D-Pen(2)-D-Pen (5)-enkephalin (DPDPE) was investigated in cell-attached patches of human neuroblastoma IMR32 cells with 100 mM Ba(2+) and 5 microM nifedipine to block L-type channels. In 70% of patches, addition of 20 microM NA + 1 microM DPDPE delayed markedly the first channel openings, causing a four- to fivefold increase of the first latency at +20 mV. The two agonists or NA alone decreased also by 35% the open probability (P(o)), prolonged partially the mean closed time, and increased the number of null sweeps. In contrast, NA + DPDPE had little action on the single-channel conductance (19 versus 19.2 pS) and minor effects on the mean open time. Similarly to macroscopic Ba(2+) currents, the ensemble currents were fast activating at control but slowly activating and depressed with the two agonists. Inhibition of single N-type channels was effectively removed (facilitated) by short and large depolarizations. Facilitatory pre-pulses increased P(o) significantly and decreased fourfold the first latency. Ensemble currents were small and slowly activating before pre-pulses and became threefold larger and fast deca...Continue Reading

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Citations

Nov 10, 2010·Cellular and Molecular Neurobiology·Kevin P M Currie
Jan 19, 2012·Journal of Molecular Neuroscience : MN·Nathalie C GuérineauEmilio Carbone
Apr 12, 2002·Progress in Neurobiology·Albertino Bigiani
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Dec 6, 2000·Immunopharmacology and Immunotoxicology·R L SciorsciP Minoia
Jun 23, 2004·Life Sciences·Shu-Shong HsuChung-Ren Jan
Oct 16, 2012·Biochimica Et Biophysica Acta·Gerald W Zamponi, Kevin P M Currie
Feb 6, 2013·Biochimica Et Biophysica Acta·David J Adams, Géza Berecki
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Dec 15, 2020·Function·Annette C Dolphin
May 16, 2021·International Journal of Cancer. Journal International Du Cancer·Teresa PecereGiorgio Palù

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