PMID: 9427254Jan 14, 1998Paper

Voltage-dependent proton transport by the voltage sensor of the Shaker K+ channel

Neuron
Dorine M StaraceF Bezanilla

Abstract

In voltage-dependent ion channels, pore opening is initiated by electrically driven movements of charged residues, and this movement generates a gating current. To examine structural rearrangements in the Shaker K+ channel, basic residues R365 and R368 in the S4 segment were replaced with histidine, and gating currents were recorded. Changes in gating charge displacement with solvent pH reveal voltage-dependent changes in exposure of the histidine to solvent protons. This technique directly monitors accessibility changes during gating, probes the environment even in confined locations, and introduces minimal interference of gating charge motion. The results indicate that charges 365 and 368 traverse the entire electric field during gating. The remarkable implication of the successive exposure of histidine to each side of the membrane is that in a pH gradient, the voltage sensor transports protons.

References

Feb 1, 1971·European Journal of Biochemistry·H Rüterjans, O Pongs
Apr 1, 1994·Biophysical Journal·E StefaniF Bezanilla
Jan 26, 1996·The Journal of Biological Chemistry·K J BoorerE M Wright
Feb 1, 1996·Neuron·H P LarssonE Y Isacoff
Nov 1, 1996·Pflügers Archiv : European journal of physiology·S P YusafA Sivaprasadarao
Mar 10, 1997·The Journal of Cell Biology·T M Shih, A L Goldin

❮ Previous
Next ❯

Citations

May 9, 2008·Cellular and Molecular Life Sciences : CMLS·T E DeCoursey
May 28, 2005·Current Opinion in Neurobiology·Mark S SondersJonathan A Javitch
May 29, 2003·Biophysical Journal·Daniel Sigg, Francisco Bezanilla
May 30, 2001·Progress in Biophysics and Molecular Biology·D Fedida, J C Hesketh
Dec 19, 2008·Nature·Kenton J Swartz
Nov 20, 2004·Nature Reviews. Neuroscience·Kenton J Swartz
Sep 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Carlos A Villalba-GaleaFrancisco Bezanilla
Feb 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Antonios PantazisRiccardo Olcese
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Carlos Gonzalez, H Peter Larsson
Apr 6, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jérôme J Lacroix, Francisco Bezanilla
Oct 19, 2011·Proceedings of the National Academy of Sciences of the United States of America·Susan M E SmithThomas E Decoursey
Aug 5, 1998·The Journal of General Physiology·B M RodríguezF Bezanilla
Oct 6, 1998·The Journal of General Physiology·A Cha, F Bezanilla
Dec 1, 1999·The Journal of General Physiology·L M Henderson, R W Meech
Feb 2, 2000·The Journal of General Physiology·S K Tiwari-WoodruffD M Papazian
Feb 2, 2000·The Journal of General Physiology·C GonzalezR Latorre
Oct 31, 2000·The Journal of General Physiology·E Loots, E Y Isacoff
Jan 3, 2001·The Journal of General Physiology·L D Islas, F J Sigworth
May 2, 2001·The Journal of General Physiology·D M Starace, F Bezanilla
Jun 29, 2001·The Journal of General Physiology·F ElinderH P Larsson
Aug 1, 2002·The Journal of General Physiology·Robert O Blaustein
Oct 3, 2002·The Journal of General Physiology·Francisco Bezanilla
Oct 3, 2002·The Journal of General Physiology·Chris S Gandhi, Ehud Y Isacoff
Oct 31, 2002·The Journal of General Physiology·Baron Chanda, Francisco Bezanilla
Nov 27, 2002·The Journal of General Physiology·Andrés MaturanaNicolas Demaurex
Nov 12, 2003·The Journal of General Physiology·Amir BroomandFredrik Elinder
Dec 17, 2003·The Journal of General Physiology·Richard Horn
Mar 17, 2004·The Journal of General Physiology·Joseph F Consiglio, Stephen J Korn
Feb 11, 2004·The Journal of General Physiology·Christopher A Ahern, Richard Horn
Jul 28, 2004·The Journal of General Physiology·Youshan YangFred J Sigworth
Nov 15, 2006·The Journal of General Physiology·Rachel B DarmanRobert O Blaustein
Jun 27, 2007·The Journal of General Physiology·Arie F Struyk, Stephen C Cannon
Apr 29, 2009·The Journal of General Physiology·Dominique G Gagnon, Francisco Bezanilla
Mar 27, 2013·The Journal of General Physiology·Kethika KulleperumaRégis Pomès

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.