Voltammetric behaviour of biological macromolecules at arrays of aqueous|organogel micro-interfaces

Physical Chemistry Chemical Physics : PCCP
Micheál D ScanlonDamien W M Arrigan

Abstract

The behaviour of two biological macromolecules, bovine pancreatic insulin and hen-egg-white lysozyme (HEWL), at aqueous-organogel interfaces confined within an array of solid-state membrane micropores was investigated via cyclic voltammetry (CV). The behaviour observed is discussed in terms of possible charge transferring species and mass transport in the interfacial reaction. Comparison of CV results for HEWL, insulin, and the well-characterised model ion tetraethylammonium cation (TEA(+)) revealed that the biomacromolecules undergo an interfacial reaction comprising biomacromolecular adsorption and facilitated transfer of electrolyte anions from the organic phase to a protein layer on the aqueous side of the interface, whereas TEA(+) undergoes a simple ion transfer process. Evidence for biomacromolecular adsorption on the aqueous side of the micro-interfaces is provided by comparison of the CVs for TEA(+) ion transfer in the presence and absence of the biomacromolecules. Similar experiments in the presence of the low generation polypropylenimine tetraamine dendrimer, (DAB-AM-4), a smaller synthetic molecule, revealed it to be non-adsorbing. The behaviour of biological macromolecules at miniaturised aqueous-organogel interface...Continue Reading

References

Mar 1, 1980·Journal of Biochemistry·S KuramitsuK Nakashima
Aug 15, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·C MattissonG Zacchi
Mar 11, 2004·Chemical Communications : Chem Comm·Damien W M ArriganValerio Beni
Jul 16, 2004·Analytical Chemistry·Dongping ZhanYuanhua Shao
Nov 27, 2004·The Journal of Pharmacy and Pharmacology·Osamu HosoyaToshinobu Seki
Feb 24, 2007·Analytical Chemistry·Jan LangmaierZdenek Samec
May 23, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Alfonso BerduqueDamien W M Arrigan
May 16, 2008·Journal of the American Chemical Society·Patrick J RodgersShigeru Amemiya
Jun 26, 2008·Analytical Chemistry·Micheál D ScanlonDamien W M Arrigan
Aug 1, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Francine KivlehanDamien W M Arrigan
Mar 24, 2009·Physical Chemistry Chemical Physics : PCCP·Micheál D ScanlonDamien W M Arrigan

Citations

Dec 15, 2012·Analytical and Bioanalytical Chemistry·Eva Alvarez de EulateDamien W M Arrigan
Mar 19, 2013·Analytica Chimica Acta·Grégoire Herzog, Valerio Beni
Dec 25, 2012·Analytical Chemistry·Shane O'Sullivan, Damien W M Arrigan
Oct 23, 2015·Angewandte Chemie·Eva Alvarez de EulateDamien W M Arrigan
Jul 26, 2012·Chemistry, an Asian Journal·Eva Alvarez de EulateDamien W M Arrigan
Dec 18, 2020·Bioelectrochemistry·Shaheda Zannah, Damien W M Arrigan

Related Concepts

Lysozyme, chicken egg white
poly(propyleneimine)
Adsorption
Bos indicus
Electric Conductivity
Electrochemistry
Electrolytes
Gel
Novolin
Leftose

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.