PMID: 9178517Jan 1, 1997Paper

Voltammetric enzyme sensor for urea using mercaptohydroquinone-modified gold electrode as the base transducer

Biosensors & Bioelectronics
Fumio MizutaniY Sato

Abstract

A voltammetric urea-sensing electrode was prepared by combining a lipid-attached urease layer with a 2,5-dihydroxythiophenol-modified gold electrode. A self-assembled monolayer of dihydroxythiophenol was prepared on the gold surface by soaking the electrode into an ethanolic solution containing the modifier. A layer of the lipid-attached enzyme and that of acetyl cellulose overcoat were successively made on the dihydroxythiophenol-modified electrode by applying a dip-coating procedure. The addition of urea in a test solution (10 mM phosphate buffer, pH 7.0) brought about an increase of pH near the urease layer. The pH shift accompanied a negative shift of the anodic peak, which corresponded to the electro-oxidation of dihydroxyphenol moiety to form quinone, on the linear sweep voltammograms for the urease/dihydroxythiophenol electrode. The concentration of urea (0.2-5 mM) could be determined by measuring the electrode current at -0.05 V versus Ag/AgCl from the voltammogram. The electrode was applied to the determination of urea in human urine; the measurement of electrode current at such a low potential provided the urea determination without any electrochemical interference from L-ascorbic acid and uric acid.

References

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Citations

May 15, 2004·Biosensors & Bioelectronics·Yung-Chien Luo, Jing-Shan Do
Oct 6, 2011·ACS Applied Materials & Interfaces·Bernd WickleinEduardo Ruiz-Hitzky
Jan 17, 2002·Bioscience, Biotechnology, and Biochemistry·A KobayashiF Mizutani
Oct 21, 2004·Biosensors & Bioelectronics·Zhiai XuShaojun Dong
Oct 6, 1999·Journal of Dairy Science·D M JenkinsR H Bondurant
Jun 14, 2020·Scientific Reports·Juanjuan LiuSebastian Wachsmann-Hogiu

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