Voltammetric evidence for discrete serotonin circuits, linked to specific reuptake domains, in the mouse medial prefrontal cortex

Neurochemistry International
Alyssa WestParastoo Hashemi

Abstract

The medial prefrontal cortex (mPFC) is an important brain region, that controls a variety of behavioral and functional outputs. As an important step in characterizing mPFC functionality, in this paper we focus on chemically defining serotonin transmission in this area. We apply cutting-edge analytical methods, fast-scan cyclic voltammetry (FSCV) and fast-scan controlled adsorption cyclic voltammetry (FSCAV), pioneered in our laboratory, for the first real-time in vivo analysis of serotonin in the mPFC. In prior in vivo work in the substantia nigra, pars reticulata, we found that our sub-second measurements of a single evoked serotonin release were subject to two clearance mechanisms. These mechanisms were readily modeled via Uptake 1, mediated by the serotonin transporters (SERTs), and Uptake 2, mediated by monoamine transporters (dopamine transporters (DATs), norepinephrine transporters (NETs), and organic cation transporters (OCTs)). Here in the mPFC, for the first time to our knowledge, we observe two release events in response to a single stimulation of the medial forebrain bundle (MFB). Of particular note is that each response is tied to a discrete reuptake profile comprising both Uptake 1 and 2. We hypothesize that two di...Continue Reading

References

Nov 22, 1991·The Journal of Comparative Neurology·R P Vertes
Mar 15, 1995·Analytical Chemistry·B P JacksonR M Wightman
Dec 26, 2001·Brain Research Bulletin·P M Whitaker-Azmitia
Oct 29, 2005·The Journal of Comparative Neurology·Trevor W Robbins
Nov 26, 2008·The Journal of Comparative Neurology·Paul J GasserChristopher A Lowry
Feb 13, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Leslie A SombersR Mark Wightman
Oct 16, 2009·Analytical Chemistry·Parastoo HashemiR M Wightman
Jan 6, 2010·Archives of General Psychiatry·Kazuhiko NakamuraNorio Mori
Mar 19, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Vera KiyasovaPatricia Gaspar
Dec 21, 2011·Neurobiology of Learning and Memory·Ruth TellezAlfredo Meneses
Jul 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Parastoo HashemiR Mark Wightman
Oct 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Bryan KolbRobbin Gibb
Apr 8, 2014·Journal of Neurochemistry·Kevin M WoodParastoo Hashemi
Apr 24, 2015·The Analyst·Srimal SamaranayakeParastoo Hashemi
Jun 18, 2015·ACS Chemical Neuroscience·Seth H WaltersAdrian C Michael
Jun 19, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Katherine R Brimblecombe, Stephanie J Cragg
Aug 2, 2016·Autism Research : Official Journal of the International Society for Autism Research·Yue-Ping Guo, Kathryn G Commons
Mar 18, 2017·Nature Reviews. Neuroscience·Christina K KimKarl Deisseroth
Aug 11, 2017·Analytical Chemistry·Aya AbdallaParastoo Hashemi

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Citations

Jul 30, 2020·Analytical Methods : Advancing Methods and Applications·Yangguang OuParastoo Hashemi
Sep 25, 2020·BMC Neuroscience·Janet BestMichael Reed
Apr 3, 2021·Analytical Chemistry·Marsilea A BoothMartyn G Boutelle
May 19, 2021·Journal of Neurovirology·Adam R DentonRosemarie M Booze
Nov 28, 2018·Analytical Chemistry·Melinda HerseyParastoo Hashemi
Jan 6, 2022·Journal of Neurophysiology·Jason YuenHojin Shin

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