Voltammetric immunoassay for Mycobacterium tuberculosis secretory protein MPT64 based on a synergistic amplification strategy using rolling circle amplification and a gold electrode modified with graphene oxide, Fe3 O4 and Pt nanoparticles

Mikrochimica Acta
Dan GouHui Chen

Abstract

The authors describe an electrochemical immunoassay for ultrasensitive determination of the Mycobacterium tuberculosis (MTb) secretory protein MPT64 which is an antigen for early diagnosis of infection with MTb. Protein G was used to immobilize antibodies against MPT64 on a gold electrode. Graphene oxide with its large surface area was used as a carrier to anchor magnetite (Fe3O4) and platinum (Pt) nanoparticles. The nanocomposite of type GO@Fe3O4@Pt was used as a signal reporter with excellent catalytic activity and recyclability. The nanocomposite exhibits peroxidase-like activity for hydrogen peroxide, best at -0.25 V (vs. Ag/AgCl). It works over a wide range of pH values (2-10) and temperatures (25-65 °C). Further signal amplification strategy was accomplished by rolling circle amplification. This voltammetric assay has a linear response to the logarithm of MPT64 concentration in the range from 5.0 fg·mL-1 to 1.0 ng·mL-1 and a detection limit of 0.34 fg·mL-1 (at a signal to noise ratio of 3, for n = 10). The assay can be completed within 4 h. It was successfully applied to the determination of MPT64 in spiked serum samples. Conceivably, the assay has a large potential in providing laboratory evidence for rapid diagnosis of ...Continue Reading

References

Sep 30, 2006·The Lancet Infectious Diseases·Karen R SteingartMadhukar Pai
Jul 26, 2008·Nature Nanotechnology·Lizeng GaoXiyun Yan
Sep 10, 2013·Tuberculosis·Neus CaceresPere-Joan Cardona
Jan 15, 2016·Applied Immunohistochemistry & Molecular Morphology : AIMM·Manju R PurohitTehmina Mustafa
Mar 16, 2017·Advanced Materials·Nargish ParvinDan Wang
Oct 4, 2017·Biosensors·Meiirbek IslamovLuis Rojas-Solórzano
Oct 24, 2017·Nature Communications·Jolien DendoovenChristophe Detavernier

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Citations

Nov 22, 2019·Mikrochimica Acta·Ramin PourakbariBalal Khalilzadeh
Sep 15, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Parinaz BabaieMohammad Hasanzadeh
Apr 29, 2021·Analytical Methods : Advancing Methods and Applications·Yuan YaoHui Chen

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