Voltammetric sensor for oxidized DNA using ultrathin films of osmium and ruthenium metallopolymers

Analytical Chemistry
Amos MugweruJames F Rusling

Abstract

Films containing [Os(bpy)2(PVP)10Cl]+ and [Ru(bpy)2(PVP)10Cl]+ metallopolymers were assembled layer by layer on pyrolytic graphite electrodes to make sensors that selectively detect oxidized DNA. These films showed reversible, independent electrochemistry for electroactive Os3+/Os2+ and Ru3+/Ru2+ centers, with formal potentials of 0.34 and 0.76 V vs SCE, respectively. The combination of ruthenium and osmium metallopolymers in the films provided a catalytic Os square wave voltammetry (SWV) peak that is mainly selective for 8-oxoguanine and the detection of other oxidized nucleobases from the Ru peak. The method is applicable to measurements on DNA in solution or DNA incorporated into films. Using the Os SWV peak, 1 oxidized nucleobase in 6000 was detected. The sensor is simple and inexpensive, and the approach may be useful for the detection of oxidized DNA as a clinical biomarker for oxidative stress.

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Citations

Sep 11, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Amos MugweruAdam Wanekaya
Feb 6, 2007·Biophysical Journal·Hong XieZhiqiang Gao
Oct 30, 2013·Analytical Chemistry·Boya SongJames F Rusling
Dec 27, 2008·Bioorganic & Medicinal Chemistry Letters·Tamer NasrShigeki Sasaki
May 12, 2007·Angewandte Chemie·Osamu NakagawaShigeki Sasaki
Jan 15, 2008·Angewandte Chemie·Clinton R SouthMarcus Weck
Jul 19, 2020·International Journal of Molecular Sciences·Györgyi FerencLajos Kovács
Jun 12, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Clinton R South, Marcus Weck
Jan 27, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Raghavendra KikkeriPeter H Seeberger
Aug 23, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Alon A Gorodetsky, Jacqueline K Barton
Sep 30, 2006·Analytical Chemistry·Jifeng LiuHubert H Girault
Dec 1, 2005·Analytical Chemistry·Jifeng LiuHubert H Girault

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