Abstract
A novel hemoglobin (Hb)-coated polystyrene (PS) latex bead film was deposited on pyrolytic graphite (PG) electrode surface. In the first step, positively charged Hb molecules in pH 5.0 buffers were adsorbed on the surface of negatively charged, 500 nm diameter PS latex beads bearing sulfate groups by electrostatic interaction. The aqueous dispersion of Hb-coated PS particles was then deposited on the surface of PG electrodes and, after evaporation of the solvent, Hb-PS films were formed. The Hb-PS film electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.36 V vs. SCE in pH 7.0 buffers, characteristic of Hb heme Fe(III)/Fe(II) redox couples. Positions of Soret absorption band of Hb-PS films suggest that Hb retains its near-native structure in the films in its dry form and in solution at medium pH. The Hb in PS films was also acted as a catalyst to catalyze electrochemical reduction of various substrates such as trichloroacetic acid (TCA), nitrite, oxygen and hydrogen peroxide.
References
May 15, 1979·Biochemistry·J B MatthewF R Gurd
Apr 15, 1994·Journal of Molecular Biology·A S Yang, B Honig
Aug 10, 1993·Biochemistry·D BashfordP E Wright
Jul 15, 1995·Analytical Chemistry·A E NassarJ F Rusling
Jan 31, 1997·Biophysical Chemistry·Z Zhang, J F Rusling
May 6, 1999·Bioelectrochemistry and Bioenergetics·J Yang, N Hu
Jan 5, 2000·Bioelectrochemistry and Bioenergetics·H SunN Hu
Mar 20, 2001·Journal of Colloid and Interface Science·Liwen Wang, Naifei Hu
Aug 17, 2001·Bioelectrochemistry·R Huang, N Hu
Sep 25, 2001·Analytical Chemistry·Y Lvov, F Caruso
Feb 28, 2002·Biomacromolecules·Pingli HeGu Zhou
Sep 18, 2002·Analytical Biochemistry·He HuangGu Zhou
Aug 14, 2003·Biophysical Chemistry·Haiyun LuNaifei Hu
Sep 1, 1953·The Biochemical Journal·P GEORGE, G HANANIA
Dec 12, 2003·The Analyst·Dongfang CaoNaifei Hu
Jan 27, 2004·Biochimica Et Biophysica Acta·Li Shen, Naifei Hu
Citations
May 21, 2005·Biophysical Chemistry·Yi LiuNaifei Hu
May 26, 2007·Bioelectrochemistry·Yi-Xin Sun, Sheng-Fu Wang
Dec 23, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Yanxia XuZhike He
Nov 14, 2008·Angewandte Chemie·Zhen GuYong Chen
May 26, 2010·Physical Chemistry Chemical Physics : PCCP·Montree Sawangphruk, John S Foord
Jun 17, 2020·Soft Matter·Junhee ChoiHo-Young Kim
Jan 29, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Gabriel LogetJoëlle Rault-Berthelot
Jun 28, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Moniraj GhoshKathleen J Stebe
Mar 9, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Sanghee KimWan-Joong Kim