Voltammetric studies of hemoglobin-coated polystyrene latex bead films on pyrolytic graphite electrodes

Biophysical Chemistry
Hong Sun, Naifei Hu

Abstract

A novel hemoglobin (Hb)-coated polystyrene (PS) latex bead film was deposited on pyrolytic graphite (PG) electrode surface. In the first step, positively charged Hb molecules in pH 5.0 buffers were adsorbed on the surface of negatively charged, 500 nm diameter PS latex beads bearing sulfate groups by electrostatic interaction. The aqueous dispersion of Hb-coated PS particles was then deposited on the surface of PG electrodes and, after evaporation of the solvent, Hb-PS films were formed. The Hb-PS film electrodes exhibited a pair of well-defined, quasi-reversible cyclic voltammetric (CV) peaks at about -0.36 V vs. SCE in pH 7.0 buffers, characteristic of Hb heme Fe(III)/Fe(II) redox couples. Positions of Soret absorption band of Hb-PS films suggest that Hb retains its near-native structure in the films in its dry form and in solution at medium pH. The Hb in PS films was also acted as a catalyst to catalyze electrochemical reduction of various substrates such as trichloroacetic acid (TCA), nitrite, oxygen and hydrogen peroxide.

References

Jan 5, 2000·Bioelectrochemistry and Bioenergetics·H SunN Hu
Sep 25, 2001·Analytical Chemistry·Y Lvov, F Caruso
Sep 1, 1953·The Biochemical Journal·P GEORGE, G HANANIA
Jan 27, 2004·Biochimica Et Biophysica Acta·Li Shen, Naifei Hu

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Citations

Dec 23, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Yanxia XuZhike He
Nov 14, 2008·Angewandte Chemie·Zhen GuYong Chen
May 26, 2010·Physical Chemistry Chemical Physics : PCCP·Montree Sawangphruk, John S Foord
Jun 17, 2020·Soft Matter·Junhee ChoiHo-Young Kim
Jan 29, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Gabriel LogetJoëlle Rault-Berthelot
Jun 28, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Moniraj GhoshKathleen J Stebe
Mar 9, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Sanghee KimWan-Joong Kim

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