Volume-activated chloride currents in fetal human nasopharyngeal epithelial cells.

The Journal of Membrane Biology
Xue-Rong SunLi-Wei Wang

Abstract

Volume-activated chloride channels have been studied by us extensively in human nasopharyngeal carcinoma cells. However, the chloride channels in the counterpart of the carcinoma cells have not been investigated. In this study, volume-activated chloride currents (I(cl,vol)) were characterized in normal fetal human nasopharyngeal epithelial cells using the whole-cell patch-clamp technique. Under isotonic conditions, nasopharyngeal epithelial cells displayed only a weak background current. Exposure to 47% hypotonic solution activated a volume-sensitive current. The reversal potential of the current was close to the calculated equilibrium potential for Cl(-). The peak values of the hypotonicity-activated current at +80 mV ranged from 0.82 to 2.71 nA in 23 cells. Further analysis indicated that the density of the hypotonicity-activated current in most cells (18/23) was smaller than 60 pA/pF. Only five cells presented a current larger than 60 pA/pF. The hypotonicity-activated current was independent of the exogenous ATP. Chloride channel inhibitors ATP, tamoxifen and 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB), inhibited the current dramatically. The anion permeability of the hypotonicity-activated chloride channels was I(-)...Continue Reading

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Citations

Jan 23, 2016·The International Journal of Biochemistry & Cell Biology·Haifeng ZhangLixin Chen
Feb 8, 2018·Cellular and Molecular Life Sciences : CMLS·Jieting ZhangHsiao Chang Chan
Apr 2, 2014·Biomedical Optics Express·Daisuke MorikawaYoshihiro Ohta
Dec 19, 2019·Journal of Peptide Science : an Official Publication of the European Peptide Society·Junghee ParkTae-Hyoung Kim

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