PMID: 7535920Nov 1, 1994Paper

Volume-activated, gadolinium-sensitive whole-cell currents in single proximal cells of frog kidney

Pflügers Archiv : European journal of physiology
L Robson, M Hunter

Abstract

Stretch-activated channels (SACs) have been implicated in the control of epithelial cell volume. Such channels are generally sensitive to the trivalent lanthanide, gadolinium (Gd3+). In this study, using Gd3+ sensitivity and volume activation as indices, we have looked for ionic currents attributable to SACs using the whole-cell-patch clamp technique in freshly isolated proximal tubule cells of the frog. Hypotonic shock caused a reversible increase in whole-cell conductance, which was inhibited by Gd3+. In conjunction with this increase in conductance, cell length (measured using an optical technique) also increased. We observed two types of volume- and Gd(3+)-sensitive currents: voltage-dependent IVD and voltage-independent IVI. IVD was found in all cells, activated by depolarisation and hypotonic shock, and was inhibited reversibly by 10 microM Gd3+. The conductance did not discriminate between Na+ and K+ but was slightly anion-selective and was Ca(2+)-permeable. IVI was observed in only 50% of cells and was also inhibited by Gd3+. Although the inhibition was irreversible, it was dose-dependent, suggesting a specific effect of Gd3+ on IVI. Cells that showed IVI had a significantly higher conductance than those that did not (3...Continue Reading

References

Jun 1, 1990·Pflügers Archiv : European journal of physiology·M Hunter
Feb 1, 1990·Pflügers Archiv : European journal of physiology·K Kawahara
Apr 1, 1989·Physiological Reviews·E K Hoffmann, L O Simonsen
Jul 1, 1988·Pflügers Archiv : European journal of physiology·H Völkl, F Lang
Jan 1, 1987·The Journal of Membrane Biology·A G Lopes, W B Guggino
Jul 1, 1985·The American Journal of Physiology·W C ColeJ S Kirkaldy
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Jan 1, 1984·The Journal of Membrane Biology·Y MatsumuraG Giebisch
Jan 1, 1983·The Journal of General Physiology·W F Boron, E L Boulpaep
Aug 1, 1994·Pflügers Archiv : European journal of physiology·L Robson, M Hunter

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Citations

Oct 1, 1994·Pflügers Archiv : European journal of physiology·S SchumannJ Leipziger
Apr 2, 2009·Medizinische Klinik·Stefan BeckerAndreas Kribben
May 2, 2009·Journal of the American College of Cardiology·Andreas KribbenRaimund Erbel
Dec 22, 1998·Journal of Biomaterials Science. Polymer Edition·A S Curtis, C D Wilkinson
Apr 9, 2001·American Journal of Physiology. Renal Physiology·I D Millar, L Robson
Nov 14, 1998·The American Journal of Physiology·H L JiD J Benos

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