Volume measurement and biophysical characterization of mounds in epithelial monolayers after intracellular bacterial infection.

STAR Protocols
Effie E. BastounisJulie A. Theriot

Abstract

Mechanical forces are important in (patho)physiological processes, including how host epithelial cells interact with intracellular bacterial pathogens. As these pathogens disseminate within host epithelial monolayers, large mounds of infected cells are formed due to the forceful action of surrounding uninfected cells, limiting bacterial spread across the basal cell monolayer. Here, we present a protocol for mound volume measurement and biophysical characterization of mound formation. Modifications to this protocol may be necessary for studying different host cell types or pathogenic organisms. For complete details on the use and execution of this protocol, please refer to Bastounis et al. (2021).

References

Sep 24, 1999·The Journal of Cell Biology·J R RobbinsJ A Theriot
Feb 1, 2006·PLoS Pathogens·Mickey PentecostManuel R Amieva
Aug 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Juan C Del AlamoJuan C Lasheras
Dec 20, 2007·The Journal of Biological Chemistry·Tomas D PerezW James Nelson
Jun 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sung Sik HurShu Chien
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Feb 3, 2015·Methods in Cell Biology·Jean-Louis MartielManuel Théry
Sep 8, 2017·Molecular Biology of the Cell·Fabian E OrtegaJulie A Theriot
Oct 24, 2017·Scientific Reports·Dominik EderChristof M Aegerter
May 31, 2018·PLoS Pathogens·Cristina FarallaAnna I Bakardjiev
Jun 26, 2020·Scientific Data·Aashrith SaraswathibhatlaJacob Notbohm

❮ Previous
Next ❯

Software Mentioned

ImageJ
PIV
_ m
MathWorks
gitlab
MATLAB
readu
run
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.