Volume phase transition of bovine vitreous body in vitro and determination of its dynamics

Biomacromolecules
Toyoaki MatsuuraMasahiko Annaka

Abstract

The phase equilibrium property and structural and dynamical properties of bovine vitreous body was studied by macroscopic observation of swelling behavior and dynamic light scattering under various conditions. It was found that the vitreous body collapses into a compact state isotropically or anisotropically depending on the external conditions. The vitreous body collapses while maintaining the shape when the pH (</= 4) and the concentration of calcium ions (</= 1 mol/L) are changed, whereas it collapses along the orbital axis in a mixed solvent of methanol and water. From observations of the dynamics of light scattered by the vitreous body, intensity autocorrelation functions that revealed two independent diffusion coefficients, D(fast) = 7.8 +/- 1.5 x 10 (- 8) cm(2)/s and D(slow) = 3.8 +/- 0.60 x 10 (-9) cm(2)/s, were obtained. The diffusion coefficients were found to be statistically independent of position within a focal depth range of 1-1.5 mm. Divergent behavior in the measured total scattered light intensities and diffusion coefficients was observed as the concentration of calcium ion approached the critical threshold, 1 mol/L. Namely, a slowing down of the dynamic modes accompanied by increased "static" scattered intens...Continue Reading

References

Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J PeetermansT Tanaka
Jul 22, 1999·European Biophysics Journal : EBJ·T MatsuuraM Saishin

❮ Previous
Next ❯

Citations

Jun 16, 2005·Photomedicine and Laser Surgery
Sep 8, 2005·Photomedicine and Laser Surgery
Jul 25, 2007·Journal of Biomedical Materials Research. Part a·Emily HoMichele Marcolongo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Physical Chemistry. B
Toyoaki MatsuuraMasahiko Annaka
European Biophysics Journal : EBJ
T MatsuuraM Saishin
Advances in Colloid and Interface Science
Kristen E BremmellDavid E Dunstan
© 2022 Meta ULC. All rights reserved