Volume regulation of cultured, transformed, non-pigmented epithelial cells from human ciliary body.

Experimental Eye Research
R E YantornoM M Civan

Abstract

Electronic cell sizing has been used to measure the volume of cells suspended in isosmotic and in hyposmotic solutions of identical ionic composition. Without inhibitors, the cells displayed a regulatory volume decrease (RVD) following anisosmotic cell swelling with a time constant (tau) of 6.3 +/- 0.9 min (mean +/- S.E.). The RVD was markedly impaired by substituting gluconate for external Cl-, and tau was prolonged by: (i) reducing the chemical gradient favoring K+ loss (by elevating the external [K+] and blocking the Na. K-exchange pump), (ii) blocking the K+ channels with Ba2+, (iii) blocking Cl- channels and Cl-/HCO3 = exchange with DIDS, and (iv) removing external HCO3-. Withdrawing HCO3- may have altered the RVD either directly by inhibiting a Cl-/HCO3- antiport, or indirectly by affecting intracellular pH. The regulatory volume response of ODM Cl-2/SV40 cells is in several respects qualitatively similar to that of non-pigmented epithelial cells of the intact ciliary body. These common characteristics suggest that the cultured cells can serve as a useful model for studying solute and fluid transport across the human ciliary epithelium. The basis for the RVD is likely to be activation of separate K+ and Cl- channels, with...Continue Reading

References

Oct 11, 1988·Biochimica Et Biophysica Acta·H Gögelein
Jan 1, 1989·Biology of the Cell·C S TangM M Civan
Jan 1, 1987·The Journal of Membrane Biology·H HelbigM Wiederholt
Jan 1, 1986·Pflügers Archiv : European journal of physiology·M Wiederholt, J A Zadunaisky
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Jul 1, 1985·New Zealand Veterinary Journal·M B Orr, G Suckling
Jun 1, 1971·The Journal of Membrane Biology·A D MacknightM M Civan

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Citations

Feb 1, 1992·Experimental Eye Research·M M CivanR E Yantorno
Jan 1, 1997·The American Journal of Physiology·C H MitchellT J Jacob
Oct 16, 2001·American Journal of Physiology. Cell Physiology·J C FleischhauerM M Civan
Mar 10, 1999·The American Journal of Physiology·C H MitchellM M Civan
Aug 15, 2001·American Journal of Physiology. Cell Physiology·C W McLaughlinM M Civan
Jul 27, 2000·American Journal of Physiology. Cell Physiology·D A CarréM M Civan
Apr 1, 1994·The American Journal of Physiology·H HasegawaA S Verkman
Feb 1, 1992·The American Journal of Physiology·R E YantornoM M Civan
Mar 1, 1994·The American Journal of Physiology·B G Kennedy
Nov 14, 1997·The American Journal of Physiology·D A CarréM M Civan
Mar 1, 1995·The American Journal of Physiology·J S Adorante, P M Cala

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