PMID: 8608171Mar 1, 1996Paper

Volume-sensitive chloride channels in the primary culture cells of human cervical carcinoma

Biochimica Et Biophysica Acta
M R ShenC Y Chou

Abstract

Previous study shows volume-sensitive chloride currents are induced by hypotonicity in human cervical cancer cell lines, but not in normal cervical epithelium. To ascertain whether the preferential activation of these channels in cancer cell lines could be similarly and directly detected in cervical cancer tissues, we studied volume-sensitive chloride channels on the primary culture cells of invasive cervical carcinoma using the whole-cell patch-clamp technique. The process of regulatory volume decrease (RVD) was also studied using electronic cell sizing to measure cell volume. Results demonstrate that, in these cultured cells, RVD was mediated in part by chloride loss through the volume-sensitive Cl- channels. A small background current with a slope conductance of 0.32 +/- 0.07 nS/pF at +30 mV (n=60 cells from 10 different samples) was observed. Hypotonicity induced a fast activating and outward rectifying current which was reversed at about 0 mV, and the slope conductance at +30 mV was increased by 10-fold to 3.62 +/- 0.62 nS/pF. These effects were readily reversed by returning the cells to isotonic medium. Moreover, DIDS, NPPB, and 1,9-dideoxyforskolin, reversibly abolished the volume-sensitive Cl- currents. The EC50 require...Continue Reading

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Citations

Dec 23, 2006·Pflügers Archiv : European journal of physiology·Hai-Yan WangYasunobu Okada
Mar 27, 2004·European Journal of Pharmacology·Baohong JiangChiyoko Inagaki
Feb 3, 2005·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Shwu-Jiuan SheuJane-Fane Chen
Jan 23, 2008·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Erika Vázquez-JuárezHerminia Pasantes-Morales
Oct 20, 2006·Journal of Cellular Physiology·Daniel DiazJuan Carlos Gomora
Jan 29, 1999·Physiological Reviews·B D SchultzR J Bridges
Sep 13, 2002·American Journal of Physiology. Cell Physiology·Lixin ChenTim J C Jacob
Sep 26, 2000·American Journal of Physiology. Cell Physiology·Y M ShubaR Skryma

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