Volume-sensitive NADPH oxidase activity and taurine efflux in NIH3T3 mouse fibroblasts.

American Journal of Physiology. Cell Physiology
Martin Barfred FriisI H Lambert

Abstract

Reactive oxygen species (ROS) are produced in NIH3T3 fibroblasts during hypotonic stress, and H(2)O(2) potentiates the concomitant release of the organic osmolyte taurine (Lambert IH. J Membr Biol 192: 19-32, 2003). The increase in ROS production [5-(and-6)-carboxy-2', 7'-dichlorodihydrofluorescein diacetate fluorescence] is detectable after a reduction in the extracellular osmolarity from 335 mosM (isotonic) to 300 mosM and reaches a maximal value after a reduction to 260 mosM. The swelling-induced ROS production is reduced by the flavoprotein inhibitor diphenylene iodonium chloride (25 microM) but is unaffected by the nitric oxide synthase inhibitor N omega-nitro-l-arginine methyl ester, indicating that the volume-sensitive ROS production is NADPH oxidase dependent. NIH3T3 cells express the NADPH oxidase components: p22 phox, a NOX4 isotype; p47 phox; and p67 phox (real-time PCR). Exposure to the Ca2+-mobilizing agonist ATP (10 microM) potentiates the release of taurine but has no effect on ROS production under hypotonic conditions. On the other hand, addition of the protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA, 100 nM) or the lipid messenger lysophosphatidic acid (LPA, 10 nM) potentiates the swelling...Continue Reading

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Citations

Mar 3, 2012·The Journal of Membrane Biology·Daniel Bloch HansenIan Henry Lambert
Sep 10, 2010·Amino Acids·Daniel Bloch HansenIan Henry Lambert
Nov 14, 2008·The Journal of Biological Chemistry·Mirkka KoivusaloSergio Grinstein
Oct 24, 2008·Acta Physiologica·I H LambertS F Pedersen
Sep 11, 2010·Journal of Biomedical Science·Stephen W SchafferJunichi Azuma
May 21, 2013·Antioxidants & Redox Signaling·Ralf P BrandesKatrin Schröder
Aug 22, 2014·Acta Physiologica·I H LambertO H Mortensen
Dec 20, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Ian Henry Lambert, Daniel Bloch Hansen
Jan 8, 2009·Physiological Reviews·Else K HoffmannStine F Pedersen
May 8, 2009·American Journal of Physiology. Cell Physiology·Ian Henry LambertElse Kay Hoffmann
Mar 15, 2013·American Journal of Physiology. Cell Physiology·Jacob Bak HolmIan Henry Lambert
Apr 4, 2014·American Journal of Physiology. Cell Physiology·Ian Henry LambertPer Amstrup Pedersen
Jun 3, 2021·Animals : an Open Access Journal From MDPI·Valeria PasciuFiammetta Berlinguer

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