Volumetric imaging: a potential tool to stage upper tract urothelial carcinoma

World Journal of Urology
Alexandra GrahnMarianne Brehmer

Abstract

To investigate whether volumetric imaging of tumor vasculature can be used to phenotypically characterize advanced upper tract urothelial carcinoma, and if this technique can distinguish aggressive invasive tumors from non-aggressive superficial ones. In a pilot study, two TaG1 and two T3G3 formalin-fixed paraffin-embedded (FFPE) tumor samples were examined using the DIPCO pipeline (Tanaka et al. in Nature Biomed Eng 1(10):796-806. https://doi.org/10.1038/s41551-017-0139-0 , 2017). Briefly, punch biopsies of FFPE tumors were deparaffinized, cleared, immunolabeled with the vessel marker CD34 and imaged with a light-sheet microscope. Thereafter, the three-dimensional (3D) vasculature of the tumors was analyzed and characterized using a specialized image processing software. We found that T3G3 tumors had increased CD34 density kurtosis and skewness compared to TaG1 tumors. This suggests that analysis of the 3D vasculature can distinguish between high-grade invasive and low-grade superficial tumors. Volumetric imaging of tumor samples may represent novel methodology that can complement conventional histopathology. Volumetric imaging enabled us to differentiate between invasive and non-invasive upper tract urothelial carcinoma. The ...Continue Reading

References

Apr 9, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kuniaki NakanishiToshiaki Kawai
Feb 29, 2008·International Journal of Urology : Official Journal of the Japanese Urological Association·Hung-Lung KeWen-Jeng Wu
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
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May 1, 2016·World Journal of Urology·Harveer S DevSamih Al-Hayek
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Apr 8, 2017·Scandinavian Journal of Urology·Camilla MalmMarianne Brehmer
Feb 3, 2018·Minerva urologica e nefrologica = The Italian journal of urology and nephrology·Angelo TerritoAlberto Breda
Feb 21, 2018·World Journal of Urology·Marianne Brehmer, UNKNOWN International Collaboration in Endourology (ICE)
Oct 1, 2017·Nature Biomedical Engineering·Nobuyuki TanakaPer Uhlén

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Citations

Sep 9, 2019·World Journal of Urology·Marianne Brehmer, Palle Osther

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Methods Mentioned

BETA
biopsies
biopsy

Software Mentioned

DIPCO
Amira

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